Effects of Metformin on experimental Varicocele in rat

Document Type: Original Articles

Authors

1 Department of Clinical Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

The aim of the current study was to determine effect of the Metformin (MET) on histopathologic evaluation and antioxidant enzyme activity in experimental varicocele-induced rat. Sixty rats were randomly divided into 6 experimental groups. The group 1 (control) had no received any medications and surgery. The group 2 (Sham) had no received any medications, abdominal cavity was opened but no varicocele-induced. The group 3 (Varicocele): abdominal cavity was opened, Varicocele-induced and no any medications applied. Group 4 abdominal cavity was opened; Varicocele-induced and animal received 25mg/kg of MET for 42 days. The groups 5 and 6 were similar to group 4, except animals received 50 and 100 mg/kg of MET, respectively. At the end of the days 21 and 42, rats were euthanized and left testis was removed for histological analysis and superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GPx) and total antioxidant status (TAS) levels. According to the results, dose dependent difference detected on testis damage grade in MET treated groups compared with varicocele group (P<0.05). No difference observed between 25 and 50 mg/kg of the MET (P>0.05). Tissue MDA levels significantly increased in varicocele rat (P<0.05) while MET (25, 50 and 100 mg/kg) in a dose dependent manner decreased varicocele-induced MDA (P<0.05). Experimental varicocele significantly decreased SOD activity compared to control group (P<0.05). Administration of the MET (25, 50 and 100 mg/kg) significantly increased tissue SOD activity in varicocele rat (P<0.05). MET (25, 50 and 100 mg/kg) in a dose dependent manner increased GPx activity in varicocele rat (P<0.05). No difference observed on MDA, SOD and GPx levels between 25 and 50 mg/kg groups (P>0.05). These findings suggested MET treatment had benefit effect against varicocele

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