Proteome analysis of toxic fractions of Iranian Cobra (Naja naja Oxiana) snake venom by 2D Electrophoresis and Mass spectrometry

Document Type: Original Articles

Authors

1 Department of Proteomics-Biochemistry, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

2 Department of Human Bacterial vaccine ,Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

3 Department of Venomous animal, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

Snake venom depending on their geographical source includes a variety of protein and peptides with toxicity and pharmacological effects. Naja naja oxiana is one the medically important venomous snake in Iran and central Asia. The bite of this type can cause severe hurt and swelling, along with neurotoxicity. Without medical treatment, symptoms rapidly worsen and death can occur soon. Understanding of venom components in details can open a new sight of making anti-venom against the toxic agents instead of crude venom.
Due to the importance of specific antibodies against toxic fractions in neutralizing the crude venom, the proteome profile of these fractions of Naja naja oxiana venom was analyzed using fractionation by gel filtration, two dimensional electrophoresis, mass spectrometry and data mining.
Base on the results, entirely 32 spots were detected and categorized into three protein family, three finger toxin (3FTx), phospholipase and Cysteine-rich secretory proteins (CRISP). These proteins are constituted more than 70% crude venom, all with a molecular weight below 25 kDa. 3FTx as a highly diverse constituent in the venom of Naja species was in large quantity in this district. Short chain neurotoxins including short neurotoxin, cytotoxin and muscarinic toxin like protein were in abundance respectively.
To sum up, revealing of proteome from toxic fractions of Naja naja oxiana from this region could provide an approach of producing an effective antivenom against similar composition and also help the medical care department to find out the clinical sign of cobra venom. To the extent of our knowledge, this the first report of proteomic of toxic fractions of Naja naja oxiana from Iran

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