Effect of Carum copticum nano essence against Saprolegnia and Fusarium and Multiplex PCR assay for the detection of these organisms in rainbow trout Oncorhynchus mykiss

Document Type: Original Articles

Authors

1 Department of Food Science and Technology, Islamic Azad University, Science and Research Branch, Tehran, Iran

2 Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

The aim of this study was to investigate the fungal species isolation and confirmation by multiplex PCR method in aquatic fish. Evaluation of the inhibitory effect of nano essential oils of Carum copticum on isolated fungal species. PCR results showed that 3 out of 5 samples were diagnosed with Fusarium solani and two of them were positive for saprolgenia. In 0.1% females' nanoparticles, one peak appeared that showed a particle with an average diameter of 360 nm, and two nanoparticles showed a peak with a mean diameter of 242 nm. The results of MIC and MFC measurements showed that nano essential oil (0.01%) had MIC with 0.08 mg/ml concentrations in nano essential oil (0.1%) with MIC with 0.04 mg concentration. Gram/ml was on the growth of Fusarium solani species. The essential oils of female plants had MIC with concentrations of 0.07 in 0.1% essential oil and 0.03 mg / ml in 0.01% oil in saprolegnia. Based on the results of MFC Nano essential oil (0.1%) on Fusarium in the range of 0.1 mg/ml on Saprolgenia 0.07 mg/ml and essential oil (0.01%) It was not found on Fusarium and Saprolegnia. Overall, the results of this study using PCR for direct detection showed that 70% of samples were Fusarium solani and 50% were Saprolgenia positive, so PCR was an efficient method for detection of fungi. Fusarium solani and Saproligenia were evaluated. According to the results of nano-essential oil (0.1%) of females, it had a strong inhibitory effect on Fusarium solani and Saprolgenia.

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