Molecular characterization and phylogenetic analysis of pathogenic Theileria spp. isolated from cattle and sheep in Iran based on cytochrome b gene

Document Type: Original Articles

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Parasitic Vaccines Research and Production, Razi Vaccine and Serum Research institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran

Abstract

In the present study, based on cytochrome b gene sequences, the phylogenetic relationship between pathogenic Theileria species in Iran including Theileria annulata and Theileria lestoquardi along with other data available in Genbank was discussed. A total of 136 (cattle) and 80 (sheep) blood samples suspected to piroplasm infection were obtained from six different provinces of Iran. Both microscopic and molecular method using species-specific primers were used for screening of T. annulata and T. lestoquardi positive samples. Finally, the partial cytochrome b gene of 30 T. annulata and 5 T .lestoquardi were amplified, sequenced and deposited in GenBank. The results indicated that there are 12 different genotypes among T. annulata isolates while only one genotype was seen among T. lestoquardi isolates. Theileria lestoquardi infection in cattle was detected in one sample and no T. annulata and T. lestoquardi co-infection was detected in sheep and cattle. In the phylogenetic tree, different Theileria species were placed in separate clades and the reliability of depicted tree and monophyly of T. annulata and T. lestoquardi ingroups were supported by bootstrap value of 94% which significantly indicate these two species evolved from a common ancestor. The tree was also showed these two pathogenic species share a more recent common ancestor with each other than with another species of Theileria parasites. To the best of our knowledge, this study is the first phylogenic analysis of pathogenic Theileria species in Iran based on cytochrome b gene sequences. In addition, the first T. lestoquardi cytochrome b gene was sequenced and deposited in GenBank.

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