Investigating the effect of sodium cyanide-induced tissue hypoxia on reproductive capability of male mice and the protective effect of ethyl pyruvate

Document Type: Original Articles


1 Genomics Division, Systems Biology Institute, Tissue Engineering Division, Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

2 Chemical Injuries Research Center, Systems Biology and Poisonings Institute Baqiyatallah University of Medical Sciences, Tehran, Iran

3 Professor of Histology and Embryology, Department of Basic Science, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

4 Assistant Professor of Histology, Vaccine & Serum Research Institute, Agricultural Research, Educational and Extension Organization (AREEO), Tehran, Iran


Decrease in oxygen delivery can lead to disturbance in testes’ functionality and spermatogenesis. The aim of this study is to examine the effect of tissue hypoxia induced by Sodium cyanide (NaCN) on fertility of male mice and the protective role of Ethyl pyruvate (EP). Therefore, a number of 30 adult mice weighing 25 to 30 grams were studied in 3 groups. The control group was treated with normal saline while another group was treated with Intraperitoneal injection (IP) of 2 mg/kg of sodium cyanide (NaCN) and the last group was treated with 2 mg/kg of sodium cyanide with an additional dose of 40 mg/kg ethyl pyruvate (NaCN+EP). The treatments were daily and continued for 35 days. After the prescription period and anesthetization, cardiac blood sampling and euthanasia were executed. Blood serum samples were provided for serum examinations (MDA, TAOC, Testosterone levels), sperm specimens were provided for sperm examinations and IVF tests, and tissue samples -after extraction and fixation in Bouin's solution, paraffin embedding and Hematoxylin and Eosin (H&E) Staining- were Consider to Histomorphometry study. The results showed significant decrease in sperm quality and reproduction potency, decrease in RI, TDI and SI factors and Leydig cells, decrement of anti-oxidant potential and increase in lipid peroxidation in sodium cyanide group (P <0.05). This is while the use of Ethyl pyruvate as a strong anti-oxidant could effectively restrain disastrous effects of tissue hypoxia. Therefore, it can be concluded that Ethyl pyruvate can moderate the complications resulted from tissue hypoxia related to testes’ parameters.