Seroepidemiological analysis of Leptospiral infection in stray dogs by MAT from rural communities of koohsar, Alborz, Iran

Document Type: Short Communication


1 Department of Biology, Science and Research Branch, Islamic Azad University,Tehran, Iran

2 Department of Microbiology, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran


Leptospirosis is a zoonosis disease that have of global importance and animals are the source of transmission of this disease through shedding in their urine. The disease caused by pathogenic Leptospiral serovars. Leptospirosis has recently been recognized as an emerging public health problem. Because of the close relationship between dogs and humans, they may play an important role in the transmission of infection to humans. The epidemiological study of leptospirosis is very important in order to find this disease in dogs and reduce the risk of transmission to humans. The aim of this study was to seroepidemiological analysis of Leptospiral infection in stray dogs by Microscopic Agglutination Test (MAT) from rural communities of koohsar, Alborz, Iran. One hundred and ten blood samples from stray dogs in Koohsar in Alborz province were collected to detect antibodies against leptospira intrrogans serovars by MAT. The prevalence of positive MAT tests in stray dogs were 21.84% (n=24). Our results demonstrated that the positive titers were ranged 1:100 to 1:1600. The following protocol confirmed that the most common titers were 1:200 (50%), 1:400 (25%), and 1:100 (12.5%). At the end, the most prevalent Leptospira serovars were L.Canicola (33.33%), L.Pomona (4.1%). No significant difference between age and sex of dogs and their MAT titer was observed (p-value ˃ 0.05). The results showed that there is high prevalence of leptospirosis in stray dogs of Koohsar in Alborz province. Since leptospirosis is a zoonosis disease, therefore the disease in humans and animals (especially dogs) should be studied continuously.