The composition and anti-toxicicity effects of Cichorium intybus distillate on serum antioxidant status in CCl4-treated female rats

Document Type: Original Articles


1 Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

2 Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran


The role of oxidative stress in female fertility is a compelling area for investigation. Cichorium intybus, known as Kasni, is believed to improve fertility according to folk medicine. For this purpose, the effects of C. intybus distillate (CI) on blood antioxidant status was investigated in rats with carbon tetrachloride (CCl4) induced toxicity. The rats were divided into four experimental groups of 10 each; Control, CI, CCl4 and CI+CCl4. The level of antioxidant enzymes activities such as glutathione peroxidase (GPx), glutathione reductase and catalase, as well as lipid peroxidation and reduced glutathione (GSH) level were measured in serum samples. In the second part of the study, the antioxidant activity and phytochemical composition of the hydrodistillate of the aerial parts of C. intybus were determined by DPPH radical scavenging and GC-MS analysis respectively.
Administration of CCl4 decreased the enzyme activities of GPx, GR and CAT which were ameliorated significantly after CI administration. The decreased level of serum GSH following CCl4 administration was not considerably elevated in CI+CCl4 group. Furthermore the level of MDA in the serum of CI+CCl4 rats was decreased compared to CCl4 group. The main compositions of the essential oil from the C. intybus distillate are antioxidants of Pulegone (8.10%), Piperitenone (7.68%), dihydroactinidiolide (5.0%) and carvone (4.18%). The antioxidant activity of the distillate, evaluated by the DPPH test was 75µg /l.
Altogether, our results demonstrated that C. intybus distillate, as a safe herbal remedy, can attenuate CCl4 induced oxidative damages which might be via boosting the endogenous antioxidant defense system.