Acaricidal activity of extract from Colchicum autumnale (autumn crocus) against Hyalomma spp. in vitro

Document Type: Original Articles


1 Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

2 Department of Basic science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran


Hyalomma spp. is responsible for transmission of protozoan, bacterial, rickettsial and viral diseases and causing economic losses to livestock industry. Recently, there is a wide number of promising attempts to evaluate and use of herbal preparations for the control of ticks. The aim of this study is to evaluate arcaricidal activity of aqueous and ethanol extracts of Colchicum autumnale rhizome and leaf against the Hyalomma spp. in vitro. The arcaricidal activities of the Colchicum leaf aqueous (CLA), leaf ethanolic (CLE), rhizome aqueous (CRA) and rhizome ethanolic (CRE)
extracts were evaluated at concentrations of 50, 100 and 150 mg/ml and controls (distilled water and Cypermethrin) following 0.25, 0.5, 0.75 and 1 h of exposure. The experiments were performed in triplicate and the experiments were performed spraying method.
Data were analyzed by GraphPad Prism 5 software. The chemical composition of aqueous leaf extract was analyzed by gas chromatography–mass spectrometry (GC–MS). The major chemical constituent was identified as Carbamodithioic acid (30.04%).
Results showed that CLA, CLE, CRA and CRE extracts have the acaricidal effect, but CLE (Colchicum leaf ethanolic) had more potent arcaricidal effects. Colchicum leaf ethanolic (CLE) extract at 50, 100 and 150 mg/ml concentration after 1 h of exposure time showed 100% mortality rate. Effectiveness of rhizome aqueous extracts (CRA) on the Hyalomma spp. was very low. The median lethal concentration (LC50) values was 100 mg/ml.
The results indicated that C. autumnale leaves contains potent acaricidal ingredients it may provide new acaricidal compounds for the effective control of Hyalomma spp. however, further studies are required to evaluate the efficacy of C. autumnale in vivo.