The potential protective role of Cyrtopodion Scabrum extract on the antioxidant parameters in serum and liver of rat with 5-FU-induced oxidative damage

Document Type: Original Articles

Authors

1 Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

2 Research Center for Traditional Medicine and History of Medicine, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

3 Razi VAaccine and Serum Research Institute Shiraz Branch, Agricultural Research, Education and Extension Organization (AREEO), Shiraz Iran

4 Department of Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

Chemotherapy is the main approach for the treatment of cancer but is often causes unpleasant oxidative damages. Therefore developing an effective alternative/complementary therapy with improved tumor suppression efficiency and lower adverse effects is highly required. Recently we showed that Cyrtopodion Scabrum extract is an effective and selective tumor suppressor medicine. In this study, the antioxidant activity of Cyrtopodion Scabrum homogenate (CsH) and extract (CsE), and their effects on attenuating 5-FU-induced liver dysfunction in rat was investigated. 60 Male rats (200-220 gr) were divided into six groups and treated for 14 days. The control (group I) and 5-FU (group II) groups were received distilled water and 5-FU, respectively. Four groups were administered by CsE, CsH, CsE+5-FU and CsH+5-FU (groups III to VI), orally by gavages in a daily schedule. 5-FU-induced oxidative damage was evaluated by changes in the weight, food and water intake during treatment and the antioxidant parameters in the liver and serum of the treated rats. Data indicated that the administration of CsH and CsE improved liver function and antioxidants defense system significantly by attenuating the levels or activities of MDA, Superoxide anion, AST, ALT, ALP and decrease of SOD, CAT, GPx, GR, GST, TAC, GSH, TP, and Alb in the liver and serum, induced by 5-FU treatment. Our data suggests that CsH and CsE play a protective role against the imbalance elicited by 5-FU and can be used as alternative/complementary supplement with 5-FU to reduce oxidative damages which is the consequence of ROS (Reactive oxygen species) production in cancerous patients.

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