Hemagglutinin-neuraminidase Sequence and Phylogenetic Analysis of Two Newcastle Disease Virus Isolated from Chickens in Iran

Document Type : Original Articles

Authors

1 Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Clinical Science, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran

3 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

Newcastle disease is a highly contagious viral infection affecting many species of birds that can spread fast between poultry houses and cause a heavy economic burden on the poultry industry all around the world. Fusion and hemagglutinin-neuraminidase (HN) protein are important in the pathogenesis of the Newcastle disease virus (NDV). The HN protein is a critical viral protein with multiple functions and plays a key role in the formation of the virulence of NDV. Head of HN protein is responsible for receptor binding, neuraminidase activity. This study aimed to investigate the sequence homology of hemagglutinin-neuraminidase of two NDV isolates sampled from infected farms in Iran. The samples were collected from flocks that had been vaccinated by both types of live and killed vaccines for NDV. After isolation of NDV, the viruses were subjected to the polymerase chain reaction (PCR) amplification using two pairs of specific primers designed for the HN gene to amplify the complete HN gene (1730bp). Afterward, the PCR products were sequenced and analyzed by phylogenetic tree construction software. Based on the analysis, substantial sequence homology among Iranian isolates is within the range of 97.1-100%. Moreover, the sequence homology searching revealed a level of similarity between HN sequences of Iranian isolates and the HN sequences from other countries, particularly Asian ones. For instance, a high homology ratio (95.34%) was found between Iranian isolates and the sequences registered on online molecular databases from China. Based on phylogenetic analysis, the NDV isolates belong to the VIId genotype. Finally, it can be concluded that monitoring the circulation of NDVs among poultry and other birds can help to obtain an insight into the evolution of NDVs and control of panzootic viruses in the future.

Keywords

Article Title [French]

Séquence de L'hémagglutinine-neuraminidase et Analyse Phylogénétique de Deux Virus de la Maladie de Newcastle Isolés de Poulets en Iran

Abstract [French]

La maladie de Newcastle est une infection virale très contagieuse affectant de nombreuses espèces d'oiseaux qui peut se propager rapidement entre les poulaillers et causer un lourd fardeau économique à l'industrie avicole du monde entier. La fusion et la protéine hémagglutinine-neuraminidase (HN) sont importantes dans la pathogenèse du virus de la maladie de Newcastle (NDV). La protéine HN est une protéine virale critique avec de multiples fonctions et joue un rôle clé dans la formation de la virulence du NDV. La tête de la protéine HN est responsable de la liaison aux récepteurs, de l'activité de la neuraminidase. Cette étude visait à étudier l'homologie de séquence de l'hémagglutinine-neuraminidase de deux isolats de NDV prélevés dans des fermes infectées en Iran. Les échantillons ont été prélevés dans des troupeaux qui avaient été vaccinés par les deux types de vaccins vivants et tués contre le NDV. Après isolement du NDV, les virus ont été soumis à l'amplification par réaction en chaîne par polymérase (PCR) en utilisant deux paires d'amorces spécifiques conçues pour le gène HN afin d'amplifier le gène HN complet (1730pb). Ensuite, les produits de PCR ont été séquencés et analysés par un logiciel de construction d'arbres phylogénétiques. Sur la base de l'analyse, une homologie de séquence substantielle parmi les isolats iraniens est comprise entre 97.1 et 100%. De plus, la recherche d'homologie de séquence a révélé un niveau de similitude entre les séquences HN des isolats iraniens et les séquences HN d'autres pays, notamment asiatiques. Par exemple, un rapport d'homologie élevé (95.34%) a été trouvé entre les isolats iraniens et les séquences enregistrées sur les bases de données moléculaires en ligne de Chine. Sur la base d'une analyse phylogénétique, les isolats de NDV appartiennent au génotype VIId. Enfin, on peut conclure que la surveillance de la circulation des NDV parmi les volailles et autres oiseaux peut aider à obtenir un aperçu de l'évolution des NDV et du contrôle des virus panzootiques à l'avenir.

Keywords [French]

  • Hémagglutinine-neuraminidase
  • Iran
  • Virus de la maladie de Newcastle
  • Arbre phylogénétique

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Archives of Razi Institute
Volume 76, Issue 1
March 2021
Pages 31-39

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