Anti-inflammatory activity of Caspian Cobra (Naja naja oxiana) snake Venom on serum level of IL-27 and Histopathological Changes in MOG-EAE induced mice

Document Type: Original Articles


1 Department of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran

2 Department of Venomous Animals and Anti venom Production, Razi Vaccine and Serum Research Institute, Agriculture Research, Education and Extension Organization, Karaj, Iran

3 Dean Faculty of Biological Sciences, Department of Biology, Kharazmi University of Tehran, Tehran, Iran


Multiple sclerosis (MS) is considered as chronic disease of the Central Nervous System (CNS), with a strong neurodegenerative component. The exact mechanism of MS is not clear. However the therapeutic strategies of controlling MS are through immune modulation and inflammation control. In this regard, the present study is to elucidate the influence of snake venom on suppressing of immune system after induction of experimental autoimmune encephalomyelitis(EAE). For this purpose, C57BL/6 female mice, divided into 3 groups, were selected—in order to be induced by EAE, the groups 2 and 3 of which were received flank injection emulsion of myelin oligodendrocyte glycoprotein (MOG 35-55) as well as complete Freund adjuvant (CFA), followed by administration of pertussis toxin(PTX). The treatment group, as an immune-modulator, also received cobra snake venom (CV) after EAE induction. Mice were then evaluated daily by dint of clinical symptoms, weight changes (within 26 days), histopathological analysis, and serum levels of IL-27 for neurological motor deficits. Clinical signs of MOG-EAE in C57BL/6 mice began between day 9-14 post-immunization. The histopathological results also revealed that CV-treated EAE mice, compared to the untreated EAE group, witnessed a significant reduction in the intensity of inflammatory cells in parenchymal sections. Furthermore, increased levels of IL-27 was significant in the CV-treated group (p=0.001) compared with the EAE and control groups. Hence based on results obtained in present study, it may be concluded that Naja naja oxiana snake venom can be as a potential candidate for considering as immunomodulatory and may be employed for MS treatment.