Effect of Taxus baccata L. Extract on Hydatid Cyst Protoscolices In vitro

Document Type : Original Articles

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

2 Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

Abstract

Hydatidosis is the most important global parasitic infectious disease both in humans and animals, which can lodge at different organs of the host, such as liver, lung (even heart), and brain which may lead to death. Surgery is the main method for the treatment of hydatidosis. In surgical therapy of hydatidosis, the use of sporicidal agents is very important since these agents inactivate live protoscolices and prevent recurrence of infection. Presently, numerous scolicidal chemical agents have been administrated to inactivate the hydatid cyst contents. Recently, there has been a high tendency among researchers to evaluate and present herbal plants as alternative option due to inexpensiveness, availability, low side effects, and toxicity. This study aimed to evaluate the scolicidal effect of hydro alcoholic Taxus baccata L. extract in vitro for the first time. The scolicidal activities of the extract were tested in concentrations of 50, 100, and 150 mg/ml following 10, 30, and 60 min of incubation, and the experiments were performed in triplicate. Viability of protoscolices was confirmed by 0.1% eosin vital staining. The data were analyzed in SAS software (version 9.4). The results showed that the hydroalcoholic extract of Taxus baccata L. at the concentration of 150 mg/ml led to killing 66.6% of protoscolices at 60 min. according to the results of this investigation, it is recommended to use this plant as a scolicidal plant. The findings of the present study showed that Taxus baccata L. had potent scolicidal effects. However, further studies are required to evaluate the efficacy of Taxus baccata L. in vivo.

Keywords

Article Title [French]

Effet de l'extrait de Taxus baccata L. sur les protoscolices de Kystes Hydatiques In vitro

Abstract [French]

L'hydatidose est la maladie infectieuse parasitaire mondiale la plus importante chez les humains et les animaux, qui peut se loger dans différents organes de l'hôte, tels que le foie, les poumons (même le cœur) et le cerveau, entraînant parfois la mort. La chirurgie est la principale méthode de traitement de l'hydatidose. Dans la thérapie chirurgicale de l'hydatidose, l'utilisation d'agents sporicides est très importante car ces agents inactivent les protoscolices vivants et préviennent la récurrence de l'infection. Actuellement, de nombreux agents chimiques scolicides ont été administrés pour inactiver le contenu du kyste hydatique. Récemment, les chercheurs portent une attention particulière aux plantes médicinales comme traitement alternatif en raison de leur faible coût, de leur disponibilité, de leurs faibles effets secondaires et toxicité. Cette étude visait à évaluer pour la première fois l'effet scolicide de l'extrait hydro-alcoolique du Taxus baccata L. in vitro. L’ activité scolicide de l'extrait a été testée à des concentrations de 50, 100 et 150 mg/ml après 10, 30 et 60 minutes d'incubation, et les expériences ont été réalisées en triple. La viabilité des protoscolices a été confirmée par une coloration vitale à l'éosine à 0,1%. Les données ont été analysées par le logiciel SAS (version 9.4). Les résultats ont montré que l'extrait hydroalcoolique du Taxus baccata L. dans une concentration de 150 mg/ml a éliminé 66,6% des protoscolices à 60 min. Selon les résultats de cette étude, l’utilisation de cette plante comme un agent scolicide est recommandée. Ces résultats montrent que l’extrait de Taxus baccata L. possède un puissant effet scolicide. Cependant, d'autres études sont nécessaires pour évaluer l'efficacité de Taxus baccata L. in vivo.

Keywords [French]

  • Kyste hydatique
  • in vitro
  • Scolicide
  • Taxus baccata L

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Archives of Razi Institute

Volume 75, Issue 4 - Serial Number 4
Winter 2021
Pages 473-480

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