Design of Nano-ELISA method for serological detection of Toxoplasmosis in mouse

Document Type: Original Articles

Authors

1 Department of Pathobiology, Faculty of Veterinary , Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Proteomics, Biochemistry and Biotechnology, Razi Vaccine and Serum Research Institute, Karaj, Iran

3 Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

Toxoplasmosis is a widespread parasitic disorder that caused by protozoan parasite Toxoplasma gondii. Nowadays, nanotechnology is used for diagnose of many infectious diseases. It could be because of nanoparticles play an important role in order to accurate and fast diagnose. The purpose of this research is to create a Nano-ELISA (enzyme linked immunosorbent assay) kit with Excerted/Secreted Antigens to have a greater sensitivity and specificity than the designed ELISA kit for detection of Toxoplasmosis in mouse. At first serum was collected from 15 infected mice by Toxoplasma gondii and 15 healthy ones. Then E/S antigen is separated from parasite Tachyzoites and used for designing ELISA kit by E/S antigens. Mice sera evaluated by designed ELISA kit. Finally Serum samples are investigated through Nano-ELISA kits that designed with E/S antigen and conjugated containing gold nanoparticles. Results showed that sensitivity and specificity of designed ELISA kit are 80% and 86.66% that both improved to 93.33% with designed Nano-ELISA kit. This results show significant improvement of sensitivity and specificity when use gold nanoparticles in design of ELISA kit. Also In current studies, the operation of E/S antigen for the design of recognizable ELISA kits have always been highlighted. The results of this research show that the use of E/S antigens in the preparation of ELISA kit is very effective. This is very important especially in the lower titers of Antibody that require more accurate diagnosis. In the other hand, Nano- ELISA designed with E/S antigen can be more sensitive and specific than ELISA method for detecting Toxoplasma and also can be the basis for further studies in this field.

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