Bioactivity of EtOH and MeOH extracts of Basidiomycetes Mushroom Stereum hirsutum on Atherosclerosis

Document Type: Original Articles


1 Akdeniz University, Faculty of Science, Department of Biology, Antalya, Turkey

2 Nigde Ömer Halisdemir University, Faculty of Medicine, Department of Cardiology, Nigde, Turkey

3 Oguzeli Vocational School, Gaziantep University, Gaziantep, Turkey

4 Nigde Ömer Halisdemir University, Faculty of Medicine, Department of Medical Biology, Nigde, Turkey


Mushroom are cosmopolitan organisms living on different substrates. Mushrooms have different pharmacological properties such as antioxidant, antimicrobial and anti-inflammator thanks to many bioactive compounds. Edible and medicinal higher fungi have been used by humankind for millennia. They are collected and used directly not only for their nutritional values as a main source of food or as a part of regular diet but also for their medicinal purpose as source of powerful new bioactive compounds. Antioxidative and anti-inflammatory functions and therefore lipid lowering effects correlate with antiatherogenic effects. In this study, the total antioxidant capacity (TAS), total oxidant capacity (TOS), oxidative stress index (OSI), 1-diphenyl-2-picrylhydrazyl (DPPH) activity and antimicrobial activity of ethanolic and methanolic extracts of Stereum hirsutum (Willd.) Pers. were determined. Also the effects on atherosclerosis is discussed according to the antioxidant activity of the mushroom. TAS, TOS and OSI values of S. hirsutum were determined using Rel Assay kits. As a result of the studies, the value of TAS was calculated as 5.289±0.113 mmol/L, TOS value was 20.540±0.416 μmol/L and OSI value was. 0.389±0.012. Free radical scavenging activity was determined by DPPH method. EtOH extracts of S. hirsutum showed higher DPPH activity than MeOH extracts. EtOH extracts at a concentration of 2 mg/mL showed an DPPH inhibition of 45.84 ± 0.81 percent. Antimicrobial activities were tested on 9 standard bacterial and fungal strains (Staphylococcus aureus, S. aureus MRSA, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Candida albicans, C. krusei, and C. glabrata) with a modified agar dilution method. Extracts showed high activity against S. aureus, S. aureus MRSA and A.baumannii. In conclusion, it was suggested that S. hirsutum can be used as a natural source related to the effects on atherosclerosis due to its antioxidant and antimicrobial activities.