Molecular detection of spotted fever group Rickettsia (Rickettsiales: Rickettsiaceae) in ticks from Iran

Document Type: Original Articles

Authors

1 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

2 Department of Plant Protection, Faculty of Agriculture, University of Lorestan, Khorramabad, Iran

3 Agricultural Research, Education and Extension Organization (AREEO), Lorestan Agricultural & Natural Resources Research Center, Khorramabad, Iran

4 Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

5 Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

Abstract

Ticks are reservoir hosts of Rickettsia pathogenic to humans and domestic animals. Most pathogenic Rickettsia species of human belong to the spotted fever group (SFG). The aim of this research was to determine which tick species were infected with Rickettsia based on the genus specific 23S rRNA, 16S rRNA and gltA gene fragments. 61 tick specimens were subjected to molecular assay and 12 samples for sequencing. Phylogenetic analyses were conducted using neighbor-joining (NJ) and Bayesian Inferences (BI) methods. Argas persicus, Haemaphysalis sulcata, Ha. inermis and Hyalomma asiaticum were to be infected by spotted fever Rickettsia. SFG is the main group of Rickettsia that can be detected from three genera of ticks in Iran.
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Ticks are reservoir hosts of Rickettsia pathogenic to humans and domestic animals. Most pathogenic Rickettsia species of human belong to the spotted fever group (SFG). The aim of this research was to determine which tick species were infected with Rickettsia based on the genus specific 23S rRNA, 16S rRNA and gltA gene fragments. 61 tick specimens were subjected to molecular assay and 12 samples for sequencing. Phylogenetic analyses were conducted using neighbor-joining (NJ) and Bayesian Inferences (BI) methods. Argas persicus, Haemaphysalis sulcata, Ha. inermis and Hyalomma asiaticum were to be infected by spotted fever Rickettsia. SFG is the main group of Rickettsia that can be detected from three genera of ticks in Iran.
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Ticks are reservoir hosts of Rickettsia pathogenic to humans and domestic animals. Most pathogenic Rickettsia species of human belong to the spotted fever group (SFG). The aim of this research was to determine which tick species were infected with Rickettsia based on the genus specific 23S rRNA, 16S rRNA and gltA gene fragments. 61 tick specimens were subjected to molecular assay and 12 samples for sequencing.

Keywords