Molecular Detection of Spotted Fever Group Rickettsia (Rickettsiales: Rickettsiaceae) in Ticks of Iran

Document Type : Original Articles

Authors

1 Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

2 Agricultural Research, Education and Extension Organization (AREEO), Lorestan Agricultural and Natural Resources Research Center, Khorramabad, Iran

3 Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

4 Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

5 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

Abstract

Ticks are reservoir hosts of pathogenic Rickettsia in humans and domestic animals. Most pathogenic Rickettsia species belong to the spotted fever group (SFG). The present study aimed to determine the tick species infected with Rickettsia based on the genus-specific 23S ribosomal ribonucleic acid (rRNA), 16S rRNA, and citrate synthase (gltA) gene fragments. A total of 61 tick specimens were selected for molecular assay and 12 samples for sequencing. Phylogenetic analysis was conducted using neighbor-joining and Bayesian inference methods. Argas persicus, Haemaphysalis sulcata, Ha. inermis, and Hyalomma asiaticum were infected by spotted fever Rickettsia. The SFG is the main group of Rickettsia that can be detected in the three genera of ticks from Iran.

Keywords


Article Title [French]

Détection Moléculaire du Groupe des Fièvres Boutonneuses Rickettsia (Rickettsiales: Rickettsiaceae) chez les Tiques d'Iran

Abstract [French]

Les tiques sont des hôtes réservoirs de Rickettsies pathogènes chez les humains et les animaux domestiques. La plupart des espèces de Rickettsia pathogènes appartiennent au groupe des fièvres boutonneuses (GFB). La présente étude visait à déterminer les espèces de tiques infectées par Rickettsia en se basant sur les fragments du genre spécifique de l'acide ribonucléique ribosomique 23S (ARNr), de l'ARNr 16S et du citrate synthase (gltA). Un total de 61 tiques a été sélectionné pour l'analyse moléculaire et 12 échantillons pour le séquençage. L'analyse phylogénétique a été menée en utilisant des méthodes de neighbor-joining et d'inférence Bayésienne. Argas persicus, Haemaphysalis sulcata, Ha. inermis et Hyalomma asiaticum sont avérés être infectés par la fièvre boutonneuse Rickettsia. Les Rickettsia sont les principaux GFB détectés dans les trois genres de tiques d'Iran.

Keywords [French]

  • Rickettsia
  • tiques
  • Groupes des fièvres boutonneuses
  • Arbre phylogénétique
  • Iran
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