Improvement in the Growth and α-toxin Production of Clostridium septicum by Magnesium Sulfate

Document Type: Original Articles

Authors

1 Salim Immune Product Company, Mashhad Branch, Razi Technology Incubator, Razi Vaccine and Serum Research Institute of Mashhad, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran

2 Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran

3 Salim Immune Product Company, Mashhad Branch, Razi Technology Incubator, Razi Vaccine and Serum Research Institute of Mashhad, Agricultural Research, Education and Extension Organization(AREEO), Mashhad, Iran

Abstract

Clostridium septicum, the anaerobic toxigenic bacterium is the agent that causes dangerous disease in man and animals. There is a lethal toxin of the bacterium namely alpha toxin. The ɑ-toxin has hemolytic, necrotic and lethal activities. Today, Razi Vaccine and Serum Research Institute of Iran produced the C. septicum vaccine in the form of bacterin/toxoid. Because of some problems, the vaccine needs to improve on an industrial scale. The study is going to find an appropriate supplement to improve growth and ɑ-toxin production. Three strains of C. septicum (vaccine, NH1 and NH8 strains) were cultured in the basic vaccine media. Magnesium sulfate, Copper, Ferrous, yeast extract, and trace elements plus vitamins' solution were added to the basic vaccine media in different cultures. The effect of the ingredients on the growth was measured by a spectrophotometer and the α-toxin secretion was assayed by hemolysin test. Growth of the bacterium and α-toxin secretion were increased by Magnesium (80 mg/l) in NH8 and vaccine strains significantly. The black precipitate was difficult to dissolve in magnesium media that must be solved. Trace elements plus vitamins solution mildly influence on NH1strain growth and toxin secretion. Other supplements (Cu, Fe, yeast extract) were not showen any significant changes in the growth and α-toxin production of C. septicum. Overflowing peptone (4%) in the vaccine media, fixes essentials of proteolysis activity, allows the sufficient growth and toxin production without Cu, Fe, and yeast extract. Due to essentially of Mg for growth, extra magnesium was added for improvement of media culture. The study suggests for Magnesium addition in the C. septicum vaccine media during production procedure after precipitation solving problem.

Keywords


Article Title [French]

Amélioration de la Croissance et de la Production de Toxine α de Clostridium septicum par Magnesium Sulfate

Abstract [French]

Clostridium septicum est une bactérie toxigène anaérobie provoquant des maladies dangereuses chez l'homme et les animaux. Il existe une toxine mortelle de la bactérie, à savoir la toxine alpha. La toxine ɑ a des activités hémolytiques, nécrotiques et mortelles. Aujourd'hui, l'Institut de recherche sur les vaccins et le sérum de Razi en Iran a produit le vaccin contre C. septicum sous forme de bactérine / anatoxine. En raison de certains problèmes, le vaccin doit s'améliorer à l'échelle industrielle. L'étude va trouver un complément approprié pour améliorer la croissance et la production de ɑ-toxines. Trois souches de C. septicum (vaccin, souches NH1 et NH8) ont été cultivées dans le milieu vaccinal de base. Du sulfate de magnésium, du cuivre, des ferreux, de l'extrait de levure et des oligo-éléments ainsi qu'une solution de vitamines ont été ajoutés au milieu vaccinal de base dans différentes cultures. L'effet des différents ingrédients sur la croissance a été mesuré par un spectrophotomètre et la sécrétion de α-toxine a été testée par test à l'hémolysine. La croissance de la bactérie et la sécrétion de α-toxine ont été augmentées de manière significative par le magnésium (80 mg/l) dans le NH8 et les souches vaccinales. Le précipité noir était difficile à dissoudre dans les milieux de magnésium qui doivent être résolus. Les oligo-éléments accompagnés d’une solution de vitamines influencent légèrement la croissance de la souche NH1 et la sécrétion de toxines. Les autres suppléments (Cu, Fe, extrait de levure) n'ont montré aucun effet significatif sur la croissance et la production de l’α-toxine de C. septicum. Le surplus de peptone e (4%) dans le milieu vaccinal, fixe l’essentiel de l'activité protéolytic et permet ainsi la production de toxines sans l’ajout de Cu, Fe et l'extrait de levure. En raison rôle essentiel de Mg pour la croissance, du magnésium supplémentaire a été ajouté pour améliorer la culture des milieux. Notre étude suggère l'ajout du magnésium dans les milieux vaccinaux de C. septicum pendant la procédure de production après la résolution du problème de précipitation.

Keywords [French]

  • Clostridium septicum
  • Milieux vaccinaux
  • Toxine alpha
  • Croissance
  • Magnésium
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