Evaluation of Interleukin-10 and Pro-inflammatory Cytokine Profile in Calves Naturally Infected with Neonatal Calf Diarrhea Syndrome

Document Type: Original Articles

Authors

1 Young Researchers and Elite Club, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran

2 Department of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran

Abstract

Multifactorial neonatal calf diarrhea (NCD) is one of the main causes of mortality in calves under 1 month of age. Studies have shown that pro-inflammatory cytokines (i.e., interleukin (IL)-1β, IL-6, and tumor necrosis factor-alpha [TNF-α]) can increase in many pathophysiological conditions. In addition, IL-10 as one of the most important anti-inflammatory cytokines contributes to the inhibition of pro-inflammatory cytokines. Few studies have addressed cytokine levels in calves with NCD. Therefore, the goal of this study was to evaluate the plasma concentrations of pro-inflammatory cytokines, as well as IL-10, in the calves naturally infected with NCD syndrome. For this purpose, 87 neonatal calves were monitored for 1 month. Out of this population, 10 cases were diagnosed with NCD and studied as the case group. In addition, a control group was considered that consisted of 10 age- and gender-matched neonatal calves without any diseases. From each calf, 5 ml blood sample was collected into EDTA tubes by jugular venipuncture. The samples were then centrifuged, and the extracted plasmas were aliquoted and stored at -20 °C. Finally, the plasma concentrations of pro-inflammatory cytokines (i.e., IL-1β, IL-6, and TNF-α) and anti-inflammatory cytokine IL-10 were measured using the ELISA kits based on the manufacturer's protocols. The results showed that the plasma concentrations of pro-inflammatory cytokines were significantly higher in the case group than in the control group. However, no significant difference was found between the two groups in terms of the plasma concentrations of IL-10. This study indicates that pro-inflammatory cytokines could be used to recognize the immune system response to NCD.

Keywords


Article Title [French]

Évaluation du Profil de l'Interleukine-10 et des Cytokines Pro-inflammatoires chez les Veaux Naturellement Infectés par le Syndrome de La Diarrhée Néonatale des Veaux

Abstract [French]

La diarrhée néonatale multifactorielle (DNM) est l'une des principales causes de mortalité chez les veaux de moins d'un mois. Des études ont montré que les taux de cytokines pro-inflammatoires (c'est-à-dire l'interleukine (IL) -1β, IL-6 et le facteur de nécrose tumorale alpha [TNF-α]) peuvent augmenter dans de nombreuses conditions physiopathologiques. De plus, l'IL-10 en tant que l'une des cytokines anti-inflammatoires les plus importantes contribue à l'inhibition des cytokines pro-inflammatoires. Peu d'études ont abordé les niveaux de cytokines chez les veaux atteints de DNM. Par conséquent, l'objectif de cette étude était d'évaluer les concentrations plasmatiques des cytokines pro-inflammatoires, ainsi que d'IL-10, chez les veaux naturellement infectés par le syndrome de DNM. À cette fin, 87 veaux nouveau-nés ont été suivis pendant 1 mois. Sur cette population, 10 cas ont été diagnostiqués avec une DNM et étudiés comme groupe de cas positifs. En outre, un groupe témoin, composé de 10 veaux nouveau-nés de même âge et sexe sans aucune maladie a été également considéré. De chaque veau, 5 ml d'échantillon de sang ont été prélevés dans des tubes EDTA par ponction veineuse jugulaire. Les échantillons ont ensuite été centrifugés et les plasmas extraits ont été aliquotés et stockés à -20 °C. Enfin, les concentrations plasmatiques de cytokines pro-inflammatoires (c'est-à-dire IL-1β, IL-6 et TNF-α) et de cytokines anti-inflammatoires IL-10 ont été mesurées à l'aide des kits ELISA basés sur les protocoles du fabricant. Les résultats ont montré que les concentrations plasmatiques de cytokines pro-inflammatoires étaient significativement plus élevées dans le groupe de cas que dans le groupe témoin. Cependant, aucune différence significative n'a été trouvée entre les deux groupes en termes de concentrations plasmatiques d'IL-10. Cette étude indique que les cytokines pro-inflammatoires pourraient être utilisées pour reconnaître la réponse du système immunitaire à la DNM.

Keywords [French]

  • Diarrhée néonatale des veaux
  • Cytokines
  • Pro-inflammatoire
  • Anti-inflammatoire
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