Role of magnetic Field in the Healing of Cutaneous Leishmaniasis Lesions in Mice

Document Type: Original Articles

Authors

1 Department of Dermatology, Razi Hospital, Faculty of Medicine, Tehran University of Medical Sciences and Health Services, Tehran, Iran

2 Department of Parasitology and Mycology, Faculty of Medicine, Aja University of Medical Sciences, Tehran, Iran

3 Department of Medical Radiology, Faculty of Paramedicine, Aja University of Medical Sciences, Tehran, Iran

Abstract

Leishmaniasisis an important tropical cutaneous disease that is endemic in the Middle East, including Iran. There is no consensus on the appropriate therapeutic method, dose, and duration for this disease. The pentavalent antimonial compounds are the first-line treatments of leishmaniasis. With regard to the resistance of this disease against drugs and its treatment failure in some patients, the present study was conducted to investigate the role of alternating magnetic field (AMF) in the treatment of cutaneous leishmaniasis lesionsin mice. To this end, 45 male Balb/c mice with the age of 3-4 weeks and weight of 18-20 g were purchased from the Pasteur Institute, Tehran, Iran, to be investigated. The mice were infected with Leishmania promastigote (2×106) injected in the upper end of the tail.After 3 weeks, the animals were screened for developing lesions. Finally, 15 mice were selected and randomly divided into three equal groups of positive control (treated with the standard drug), negative control (without treatment), and experimental (subjected to AMF at a frequency of 50 kHz for 30 min on a daily basis for 4 weeks). The subjects were followed up for 4 weeks, and the size of their lesions was measured weekly. The data were analyzed by repeated measures test in SPSS software (version 20) at a p-value of < 0.05. There was no significant difference between the experimental and positive control groups (P>0.05). However, the negative control group showed a significant difference with the positive control and experimental groups (P=0.0001). As the findings indicated, AMF was seemingly able to decrease the size of lesions to the same extent as the standard drug. Consequently, AMF could be suggested as a noninvasive and complementary tool against cutaneous leishmaniasis. However, it is required to perform more studies to address different aspects of this domain.

Keywords


Article Title [French]

Rôle du Champ Magnétique dans la Cicatrisation des Lésions cutanées de Leishmaniose chez les Souris

Abstract [French]

La leishmaniose est une importante maladie cutanée tropicale endémique au Moyen-Orient, y compris en Iran. Il n'y a pas de consensus sur la méthode thérapeutique, la dose et la durée appropriées pour cette maladie. Les composés antimoniaux pentavalents sont les traitements de première ligne de la leishmaniose. Etant donné la résistance de cette maladie aux médicaments et l’échec thérapeutique chez certains patients, la présente étude a été menée pour étudier le rôle du champ magnétique alternatif (CMA) dans le traitement des lésions de leishmaniose cutanée chez les souris. Dans ce but, 45 souris Balb/c mâles âgées de 3 à 4 semaines et pesant de 18 à 20g ont été achetées à l'Institut Pasteur de Téhéran, en Iran, pour le besoin de cette étude. Les souris ont été infectées par Leishmania promastigote (2×106) injecté dans l'extrémité supérieure de la queue. Après 3 semaines, les animaux ont été examinés pour le possible développement de lésions. Enfin, 15 souris ont été sélectionnées et réparties au hasard en trois groupes égaux de contrôle positif (traités avec le médicament standard), de contrôle négatif (sans traitement), et expérimental (soumis au CMA à une fréquence de 50 kHz pendant 30 min sur une base quotidienne durant 4 semaines). Les sujets ont été suivis durant 4 semaines, et la taille de leurs lésions a été mesurée chaque semaine. Les données ont été analysées par un test de mesures répétées dans le logiciel SPSS (version 20) à une valeur de p <0,05. Il n'y avait pas de différence significative entre les groupes expérimentaux et contrôle positif (P> 0,05). Cependant, le groupe contrôle négatif a montré une différence significative avec le groupe contrôle positif et le groupe expérimental (P = 0,0001). Comme les résultats l'ont indiqué, le CMA était apparemment en mesure de réduire la taille des lésions dans les mêmes proportions que le médicament standard. Par conséquent, le CMA pourrait être suggérée comme un outil non invasif et complémentaire contre la leishmaniose cutanée. Cependant, il est nécessaire d'effectuer plus d'études pour aborder différents aspects de ce traitement.

Keywords [French]

  • Leishmaniose cutanée
  • Champ magnétique alternatif
  • Traitement
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