Molecular detection of Theileria spp. and Babesia ovis Infection in Sheep in Baneh, Iran

Document Type: Case Study

Authors

1 Department of Parasite Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Agriculture Research, Education, and Extension Organization (AREEO), Karaj, Iran

2 Student, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, Iran

Abstract

The purpose of the present study was to investigate the Theileria and Babesia infection in sheep using polymerase chain reaction (PCR) assay in Baneh, Iran. Theileria and Babesia are apicomplexan parasites that have both vertebral and invertebrate hosts. These protozoa, which are transmitted by tick vectors, are considered to be the most important causes of parasitic diseases in Iran.The detection methods of Babesia and Theileria spp. are morphological examination, serology tests, and more recently, molecular assays, such as PCR.  In this study, a total of 66 blood samples were collected and analyzed using specific primers for Theileria annulata, T. ovis, T. lestoquardi, and Babesia ovis. Two PCR methods were used, namely semi-nested PCR and competitive PCR. Based on the results of the PCR assay of 66 sheep blood samples, B. ovis, T. ovis, T. lestoquardi, and T. annulata were detected in 57 (86.4%), 28 (42.4%), 0, and 16 (24%) cases, respectively. Detection of low levels of protozoan infection with high morbidity in the tested animals shows their status as a carrier that keeps the infection in the region and extends the protozoan life cycle. Another important factor is the geographical situation of Baneh as a border city since the hemoprotozoan infection is present in this region. Moreover, piroplasmida infection was found in Iraq and other neighboring provinces. Therefore, animal husbandry in Baneh is at the risk of infection with Babesia and Theileria. The collected data in this study are useful for reaching a better understanding of the epizootiology of theileriosis and babesiosis, in order to control and prevent the diseases in this region. 

Keywords


Article Title [French]

Détection Moléculaire des Infections aux Espèces de Theileria et Babesia ovis chez les Moutons à Baneh, Iran

Abstract [French]

Le but de cette étude était de détecter les infections à Theileria et Babesia chez les moutons à l'aide du test de réaction en chaîne par polymérase (PCR) à Baneh, en Iran. Theileria et Babesia sont des parasites apicomplexes qui ont des hôtes vertébraux et invertébrés. Ces protozoaires, qui sont transmis par des tiques, sont considérés comme les causes les plus importantes de maladies parasitaires en Iran.Les méthodes utilisées pour la détection de Babesia et Theileria spp. sont l'examen morphologique, les tests sérologiques et, plus récemment, les tests moléculaires, tels que la PCR. Dans cette étude, un total de 66 échantillons de sang ont été prélevés et analysés à l'aide d'amorces spécifiques pour Theileria annulata, T ovis, T. lestoquardi et Babesia ovis. Deux méthodes de PCR ont été utilisées, à savoir la PCR semi-nested et la PCRcompétitive. Sur la base des résultats du test PCR des 66 échantillons de sang de mouton, B. ovis, T. ovis, T. lestoquardi et T. annulata ont été respectivement détectés dans 57 (86,4%), 28 (42,4%), 0 et 16 (24%) cas. La détection de faibles niveaux d'infection protozoaire avec une morbidité élevée chez les animaux testés montre leur statut de porteur qui maintient l'infection dans la région et prolonge le cycle de vie du protozoaire. Un autre facteur important est la situation géographique de Baneh en tant que ville frontalière, maintenant le risque dl'infection par les hémoprotozoaires dans cette région. De plus, une infection à piroplasmida a été trouvée en Irak et dans les autres provinces voisines. Par conséquent, les élevages de Baneh sont sujets à des risques élevés d'infections par Babesia et Theileria. Les données recueillies dans cette étude nous aiderons à parvenir à une meilleure compréhension de l'épizootiologie de la théilériose et de la babésiose dans cette région, indispensable dans le contrôler et la prévention de ces maladies.

Keywords [French]

  • Babesia
  • Hyalomma
  • Kurdistan
  • RCP
  • Theileria
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