A Study on Isolation and Molecular Identification of Bordetella avium from Iranian Commercial and Backyard Broiler Turkeys within 2016-2018

Document Type: Original Articles

Authors

1 Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

4 Department of Virology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

5 School of Integrated Sciences, James Madison University, Harrisonburg, Virginia, United States

6 Department of Medical Microbiology, Adnan Menderes University, Aydin, Turkey

Abstract

Bordetellosis or turkey coryza, caused by Bordetella avium, has been an issue for turkey industry since its first description in 1967 when it was reported for the first time. Bordetella avium causes a highly contagious upper respiratory disease in turkeys. Therefore, this study aimed to isolate and characterize this species from commercial and backyard turkeys in Tehran, Isfahan, and Northern provinces of Iran. For the purpose of the study, 625 tracheal swabs were taken from 425 commercial poults and 200 backyard poults aged 2-6 weeks from September 2016 to September 2018. The swabs were immediately plated on MacConkey and blood agar plates and then pooled (5 swabs/pool) in tubes, containing 2 mL distilled water, to perform direct polymerase chain reaction (PCR) for the identification of B. avium. A total of 17 swab pools were found to be positive for B. avium. A subset of seven positive samples were sequenced for the flanking region of piuA gene. The analysis of the sequences indicated that the sequences were 98%, 96%, and 98% similar to B. avium 197N (AM167904.1), 4142 (AY925058.1), and 4156 (AY925068.1) sequences, respectively. To the best of our knowledge, the current study is the first attempt toward the molecular detection and characterization of B. avium in Iran. It is highly recommended to perform further studies to isolate, characterize, and differentiate the regional isolates in order to help the developing turkey industry of Iran meet the increasing demands for protein in the diet of the citizenry.

Keywords


Article Title [French]

Une Étude sur l'Isolement et l'Identification Moléculaire de Bordetella avium Provenant de Dindes Commerciales et de Basse-cour Iraniennes entre 2016-2018

Abstract [French]

La bordétellose ou coryza de la dinde, causée par Bordetella avium, représente un problème pour l'industrie de la dinde depuis sa première description en 1967. Bordetella avium provoque une maladie des voies respiratoires supérieures très contagieuse chez les dindes. Par conséquent, cette étude visait à isoler et caractériser cette espèce chez les dindes commerciales et de basse-cour à Téhéran, Ispahan et les provinces du nord de l'Iran. A cet effet, 625 écouvillons trachéaux ont été prélevés sur 425 dindonneaux commerciaux et 200 dindonneaux de basse-cour âgés de 2 à 6 semaines entre septembre 2016 et septembre 2018. Les écouvillons ont été immédiatement étalés sur des plaques de MacConkey et de gélose au sang, puis regroupés (5 écouvillons/pool) dans des tubes, contenant 2 ml d'eau distillée, pour effectuer une réaction en chaîne par polymérase (RCP) directe pour l'identification de B. avium. Au total, 17 pools d'écouvillons étaient positifs pour B. avium. Un sous-ensemble de sept échantillons positifs a été séquencé pour la région flanquante du gène piuA. L'analyse des séquences a indiqué que les séquences étaient 98%, 96% et 98% similaires aux séquences de B. avium 197N (AM167904.1), 4142 (AY925058.1) et 4156 (AY925068.1), respectivement. Au meilleur de nos connaissances, cette étude représente la première tentative de détection moléculaire et de caractérisation de B. avium en Iran. Nos résultats recommandent fortement la mise em place d'autres études afin d’isoler, caractériser et différencier les isolats régionaux pour l'industrie en développement de la dinde d'Iran à répondre aux demandes croissantes de protéines dans l'alimentation des citoyens.

Keywords [French]

  • identification moléculaire
  • Bordetella avium
  • Dinde
  • L'Iran
  • Arbre phylogénétique
Abo-State, M., Riad, B., Bakr, A., Abdel Aziz, M., 2018. Biodegradation of naphthalene by Bordetella avium isolated from petroleum refinery wastewater in Egypt and its pathway. J Radiat Res Appl Sci 11, 1-9.

Beach, N.M., Thompson, S., Mutnick, R., Brown, L., Kettig, G., Puffenbarger, R., et al., 2012. Bordetella avium antibiotic resistance, novel enrichment culture, and antigenic characterization. Vet Microbiol 160, 189-196.

Blackall, P.J., Eaves, L.E., Fegan, M., 1995. Antimicrobial sensitivity testing of Australian isolates of Bordetella avium and the Bordetella avium-like organism. Aust Vet J 72, 97-100.

Blackall, P.J., Farrah, J.G., 1986. An evaluation of two methods of substrate alkalinization for the identification of Bordetella avium and other similar organisms. Vet Microbiol 11, 301-306.

Cai, H., Archambault, M., Prescott, J.F., 2003. 16S ribosomal RNA sequence-based identification of veterinary clinical bacteria. J Vet Diagn Invest 15, 465-469.

El-Sukhon, S.N., Musa, A., Al-Attar, M., 2002. Studies on the bacterial etiology of airsacculitis of broilers in northern and middle Jordan with special reference to Escherichia coli, Ornithobacterium rhinotracheale, and Bordetella avium. Avian Dis 46, 605-612.

Grespan, A., Camera, O., Knobl, T., Gomes, C.R., Felizardo, M.R., Ferreira, T.S., et al., 2012. Virulence and molecular aspects of Bordetella avium isolated from cockatiel chicks (Nymphicus hollandicus) in Brazil. Vet Microbiol 160, 530-534.

Hinz, K.H., Heffels-Redmann, U., Poppel, M., 1992. [Multicausal infectious respiratory tract disease of young fattening turkeys]. Dtsch Tierarztl Wochenschr 99, 75-78.

Jackwood, W., Saif, Y.M., 2013. Bordetellosis. In: Swayne, D. (Ed.), Diseases of poultry, Willey Blackwell, New York, pp. 834-858.

Kelly, B.J., Ghazikhanian, G.Y., Mayeda, B., 1986. Clinical outbreak of Bordetella avium infection in two turkey breeder flocks. Avian Dis 30, 234-237.

Kersters, K., Hinz, K.-H., Hertle, A., Segers, P., Lievens, A., Siegmann, O., et al., 1984. Bordetella avium sp. nov., isolated from the respiratory tracts of turkeys and other birds. Int J Syst Evol Microbiol 34, 56-70.

Malik, Y., Olsen, K., Chander, Y., Goyal, S., 2003. Antimicrobial resistance in bacterial pathogens isolated from turkeys in Minnesota from 1998 to 2002. J Appl Res Vet Med 1, 212-218.

Ozbey, G., Muz, A., 2006. Isolation of aerobic bacteria from the lungs of chickens showing respiratory disorders and confirmation of Pasteurella multocida by polymerase chain reaction (PCR). Vet Arhiv 76, 217-225.

Panigrahy, B., Grumbles, L.C., Terry, R.J., Millar, D.L., Hall, C.F., 1981. Bacterial coryza in turkeys in Texas. Poult Sci 60, 107-113.

Pumford, N., Morgan, M., Wolfenden, A., Hargis, B., Tellez, G., 2008. Isolation and prevention of Bordetella avium in Commercial turkeys. Avi De Cen De Mex 2, 72.

Register, K.B., Jackwood, L., 2016. A laboratory manual for the isolation, identification, and characterization of avian pathogens, American Association of Avian Pathologists, New York.

Register, K.B., Yersin, A.G., 2005. Analytical verification of a PCR assay for identification of Bordetella avium. J Clin Microbiol 43, 5567-5573.

Sacco, R., Register, K., Nordholm, G., 2000. Restriction enzyme analysis and ribotyping distinguish Bordetella avium and Bordetella hinzii isolates. Epidemiol Infect 124, 83-90.

Salahi, A., 2014. A review of the turkey meat production industry in Iran. Zootec Int 36, 24-29.

Savelkoul, P.H., de Groot, L.E., Boersma, C., Livey, I., Duggleby, C.J., van der Zeijst, B.A., et al., 1993. Identification of Bordetella avium using the polymerase chain reaction. Microb Pathog 15, 207-215.

Smialek, M., Tykalowski, B., Pestka, D., Stenzel, T., Koncicki, A., 2015. Epidemiological situation of turkey coryza (bordetellosis) in Poland. Pol J Vet Sci 18, 659-661.

Statistical Center of Iran, 2019. Statistical Center of Iran. www.amar.org/english.

Szabo, R., Wehmann, E., Magyar, T., 2015. Antimicrobial susceptibility of Bordetella Avium and Ornithobacterium Rhinotracheale strains from wild and domesticated birds in Hungary. Acta Vet Hung 63, 413-424.

Türkyilmaz, S., Kirdar, S., Ocak, F., Hazimoğlu, Ş., 2009. Detection of Bordetella avium by polymerase chain reaction in the lungs and tracheas of turkeys with pneumonia. Turk J Vet Anim Sci 33, 147-150.

Van Alstine, W.G., 1987. Structural and functional effects of Bordetella avium infection in the turkey respiratory tract, Iowa State University, Ames, Iowa.

Venne, D., Brugere-Picoux, J., 2015. Bordetellosis, Manual of Poultry Diseases, France.