Molecular Monitoring of D1466 Genotype of Avian Infectious Bronchitis Virus in Iran: A Retrospective Study in 2013-2017

Document Type: Original Articles


1 Iranian Veterinary Organization, Tehran, Iran

2 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Department of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization (AREEO), Karaj, Iran

4 Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran


Infectious bronchitis is one of the most common diseases in the poultry industry in many countries, especially in the regions with a dense poultry farming industry. Gammacoronavirus is the etiologic agent of this disease, with the chickens and poultries as the natural reservoirs of the virus. Various strains of infectious bronchitis virus have been reported in poultry around the world. In terms of pathogenicity, this virus can induce a spectrum of diseases ranging from the moderate respiratory tract to kidney and reproductive diseases. The serotypes of this virus do not cause cross-immunity against each other. This issue makes it difficult to control the disease. Based on the analysis of the highly variable region of the glycoprotein S1 gene, the isolated strains in Iran were classified into seven different phylogenetic groups, including Massachusetts, QX, IS-720, IS-1494, 793/B, IR-1, and IR-2. The D1466 genotype has not been reported in the country; however, the killed vaccine is used in broiler breeder farms. In this study, tissue specimens were collected from 700 farms (i.e., broiler, egg-laying, and broiler breeder farms) suspected of infectious bronchitis within 2013-2017. The samples were examined using real-time reverse transcription-polymerase chain reaction. The D1466 genotype was not detected in any of the studied specimens. Due to the lack of immunity of the D1466 serotype against the dominant types in the country, one has to be careful in choosing the right vaccine. It is necessary to perform continuous monitoring of the circulation status of the various serotypes of viruses in the country to identify the dominant and possible new serotypes for the utilization of the appropriate vaccine.


Article Title [French]

Surveillance Moléculaire du Génotype D1466 du Virus de la Bronchite Infectieuse Aviaire en Iran: une Étude Rétrospective en 2013-2017

Abstract [French]

La bronchite infectieuse est l'une des maladies les plus courantes dans l'industrie de la volaille dans de nombreux pays, en particulier dans les régions où l'industrie de l'élevage de volaille est dense. Le gammacoronavirus est l'agent étiologique de cette maladie, les poulets et les volailles étant les réservoirs naturels du virus. Diverses souches de virus de la bronchite infectieuse ont été signalées chez les volailles du monde entier. En termes de pathogénicité, ce virus peut induire un spectre de maladies allant des voies respiratoires modérées aux maladies rénales et reproductives. Les sérotypes de ce virus ne provoquent pas d'immunité croisée les uns contre les autres. Ce problème rend difficile le contrôle de la maladie. Sur la base de l'analyse de la région hautement variable du gène de la glycoprotéine S1, les souches isolées en Iran ont été classées en sept groupes phylogénétiques différents, dont le Massachusetts, QX, IS-720, IS-1494, 793/B, IR-1, et IR-2. Le génotype D1466 n'a pas été signalé dans le pays; cependant, le virus inactivé est utilisé dans les fermes d'élevage de poulets de chair. Dans cette étude, des échantillons de tissus ont été prélevés de 700 fermes (c.-à-d. les Fermes d'élevage de poulets de chair, de poules pondeuses, et de poulets reproducteurs) soupçonnées de bronchite infectieuse en 2013-2017. Les échantillons ont été examinés en utilisant une transcription inverse - réaction en chaîne par polymérase (TI-RCP) en temps réel. Le génotype D1466 n'a été détecté dans aucun des échantillons étudiés. En raison du manque d'immunité du sérotype D1466 contre les types dominants dans le pays, il faut être prudent dans le choix du bon vaccin. Il est nécessaire d'effectuer une surveillance continue de l'état de circulation des différents sérotypes de virus dans le pays pour identifier les nouveaux sérotypes dominants et possibles pour l'utilisation du vaccin approprié.

Keywords [French]

  • Bronchite infectieuse D1466
  • Fermes commerciales
  • TI-RCP en temps réel
  • Iran
Acevedo, A.M., Perera, C.L., Vega, A., Rios, L., Coronado, L., Relova, D., et al., 2013. A duplex SYBR Green I-based real-time RT-PCR assay for the simultaneous detection and differentiation of Massachusetts and non-Massachusetts serotypes of infectious bronchitis virus. Mol Cell Probes 27, 184-192.

Aghakhan, S., Abshar, N., Fereidouni, S.R.N., Marunesi, C., Khodashenas, M., 1994. Studies on Avian Viral Infections in Iran. Arch Razi Inst 44.45, 1-10.

Ahmed, Z., Naeem, K., Hameed, A., 2007. Detection and seroprevalence of infectious bronchitis virus strains in commercial poultry in Pakistan. Poult Sci 86, 1329-1335.

Casais, R., Dove, B., Cavanagh, D., Britton, P., 2003. Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism. J Virol 77, 9084-9089.

Cavanagh, D., Mawditt, K., Britton, P., Naylor, C.J., 1999. Longitudinal field studies of infectious bronchitis virus and avian pneumovirus in broilers using type-specific polymerase chain reactions. Avian Pathol 28, 593-605.

Cook, J.K., Orbell, S.J., Woods, M.A., Huggins, M.B., 1999. Breadth of protection of the respiratory tract provided by different live-attenuated infectious bronchitis vaccines against challenge with infectious bronchitis viruses of heterologous serotypes. Avian Pathol 28, 477-485.

Davelaar, F.G., Kouwenhoven, B., Burger, A.G., 1984. Occurrence and significance of infectious bronchitis virus variant strains in egg and broiler production in the Netherlands. Vet Q 6, 114-120.

Domanska-Blicharz, K., Lisowska, A., Pikula, A., Sajewicz-Krukowska, J., 2017. Specific detection of GII-1 lineage of infectious bronchitis virus. Lett Appl Microbiol 65, 141-146.

Ghalyanchi-Langeroudi, A., Karimi, V., Jannat, A., Hashemzadeh, M., Fallah, M., Gholami, F., et al., 2015. Genotyping of infectious bronchitis viruses in the east of Iran, 2015. Iran J Virol 9, 31-35.

Hosseini, H., Fard, M.H., Charkhkar, S., Morshed, R., 2015. Epidemiology of Avian Infectious Bronchitis Virus Genotypes in Iran (2010-2014). Avian Dis 59, 431-435.

Ignjatovic, J., Ashton, D.F., Reece, R., Scott, P., Hooper, P., 2002. Pathogenicity of Australian strains of avian infectious bronchitis virus. J Comp Pathol 126, 115-123.

Ignjatovic, J., Sapats, S., 2000. Avian infectious bronchitis virus. Rev Sci Tech 19, 493-508.

Jackwood, M., de Wit, S., 2013. Infectious bronchitis. In: Swayne, D. (Ed.), Diseases of poultry, Wily Blackwell, New Jersey, pp. 139-160.

Jackwood, M.W., 2012. Review of infectious bronchitis virus around the world. Avian Dis 56, 634-641.

Kusters, J.G., Niesters, H.G., Bleumink-Pluym, N.M., Davelaar, F.G., Horzinek, M.C., Van der Zeijst, B.A., 1987. Molecular epidemiology of infectious bronchitis virus in The Netherlands. J Gen Virol 68, 343-352.

Kusters, J.G., Niesters, H.G., Lenstra, J.A., Horzinek, M.C., van der Zeijst, B.A., 1989. Phylogeny of antigenic variants of avian coronavirus IBV. Virology 169, 217-221.

Lee, C.W., Jackwood, M.W., 2000. Evidence of genetic diversity generated by recombination among avian coronavirus IBV. Arch Virol 145, 2135-2148.

Lee, C.W., Jackwood, M.W., 2001a. Origin and evolution of Georgia 98 (GA98), a new serotype of avian infectious bronchitis virus. Virus Res 80, 33-39.

Lee, C.W., Jackwood, M.W., 2001b. Spike gene analysis of the DE072 strain of infectious bronchitis virus: origin and evolution. Virus Genes 22, 85-91.

Marandino, A., Tomas, G., Hernandez, M., Panzera, Y., Craig, M.I., Vagnozzi, A., et al., 2016. Development of RT-qPCR assays for the specific identification of two major genotypes of avian infectious bronchitis virus. J Virol Methods 235, 21-25.

Meir, R., Rosenblut, E., Perl, S., Kass, N., Ayali, G., Perk, S., et al., 2004. Identification of a novel nephropathogenic infectious bronchitis virus in Israel. Avian Dis 48, 635-641.

Modiri Hamadan, A., Ghalyanchilangeroudi, A., Hashemzadeh, M., Hosseini, H., Karimi, V., Yahyaraeyat, R., et al., 2017. Genotyping of Avian infectious bronchitis viruses in Iran (2015-2017) reveals domination of IS-1494 like virus. Virus Res 240, 101-106.

Moore, K.M., Bennett, J.D., Seal, B.S., Jackwood, M.W., 1998. Sequence comparison of avian infectious bronchitis virus S1 glycoproteins of the Florida serotype and five variant isolates from Georgia and California. Virus Genes 17, 63-83.

Najafi, H., Langeroudi, A.G., Hashemzadeh, M., Karimi, V., Madadgar, O., Ghafouri, S.A., et al., 2016. Molecular characterization of infectious bronchitis viruses isolated from broiler chicken farms in Iran, 2014-2015. Arch Virol 161, 53-62.

Pourbakhsh, S., Momayez, R., Toroghi, R., Shoushtari, A., 2008. Ninetythree B type, the Predominant Circulating Type of Avian Infectious Bronchitis Viruses 1999-2004 in Iran: a retrospective study. Arch Razi Inst 63, 1-5.

Roh, H.J., Hilt, D.A., Jackwood, M.W., 2014. Detection of infectious bronchitis virus with the use of real-time quantitative reverse transcriptase-PCR and correlation with virus detection in embryonated eggs. Avian Dis 58, 398-403.

Seifi, S., Asasi, K., Mohammadi, A., 2010. Natural co-infection caused by avian influenza H9 subtype and infectious bronchitis viruses in broiler chicken farms. Veterinarski Arhiv 80, 269-281.

Sjaak de Wit, J.J., Cook, J.K., van der Heijden, H.M., 2011. Infectious bronchitis virus variants: a review of the history, current situation and control measures. Avian Pathol 40, 223-235.

Tan, L., Liao, Y., Fan, J., Zhang, Y., Mao, X., Sun, Y., et al., 2016. Prediction and identification of novel IBV S1 protein derived CTL epitopes in chicken. Vaccine 34, 380-386.

Worthington, K.J., Currie, R.J., Jones, R.C., 2008. A reverse transcriptase-polymerase chain reaction survey of infectious bronchitis virus genotypes in Western Europe from 2002 to 2006. Avian Pathol 37, 247-257.