Molecular and Serological Study of Neospora caninum Infection among Dogs and Foxes in Sanandaj, Kurdistan Province, Iran

Document Type: Original Articles

Authors

1 Department of Pathobiology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran

Abstract

Neospora caninum, a protozoan parasite, causes abortions in cattle, as well as neurological disorders and reproductive problems in dogs. This study aimed to investigate the serological and the molecular prevalence of N.caninum among foxes and dogs using indirect fluorescent antibody test (IFAT) and nested-polymerase chain reaction (PCR). For this purpose, 288 and 95 both fecal and serum samples of dogs and foxes were collected, respectively, from around industrial and traditional dairy flocks in different parts of Sanandaj, Kurdistan Province, Iran, from 2013 to 2015. The sera were examined using IFAT, and fecal samples were microscopically assessed for detecting Neospora oocyst and by nested-PCR. The findings revealed that N.caninum seroprevalence were 4.86% and 4.21% in dogs and foxes, respectively. In addition, no Neospora oocysts were found microscopically and by PCR. Since this study is the first serological and molecular investigation of N.caninum among both dogs and foxes in Sanandaj,  the findings of indicated that stray dogs is a main source of N.caninum infection in dairy farms in Sanandaj, Iran. 

Keywords


Article Title [French]

Étude Moléculaire et Sérologique de Neospora caninum chez des Chiens et des Renards dans le District de Sanandaj, Province du Kurdistan (Iran)

Abstract [French]

Neospora caninum, un parasite protozoaire, provoque à la fois des avortements chez les bovins ainsi que des troubles neurologiques et des problèmes de reproduction chez les chiens. Dans cette étude, la prévalence sérologique et moléculaire de Neospora caninum a été étudiée chez les renards et les chiens par le biais de test d'immunofluorescence indirecte (IFA) et une nested-PCR. Pour cela, 288 et 95 échantillons fécaux et sériques de chiens et de renards ont été respectivement collectés entre 2013 et 2015 dans des troupeaux laitiers industriels et traditionnels dans différentes parties du district de Sanandaj, dans la province du Kurdistan. Les sérums ont été examinés avec le test IFA et par nested-PCR, alors que des échantillons de matières fécales ont été examinés au microscope pour détecter la présence d'oocyste de Neospora et. Les résultats ont révélé que la séroprévalence de N. caninum était respectivement de 4,86% et 4,21% chez les chiens et les renards. De plus, aucun oocyste de Neospora n'a été détecté au microscope et par PCR. Comme cette étude est la première enquête sérologique et moléculaire de N. caninum chez les chiens et les renards de Sanandaj, les résultats de l'étude ont indiqué que les chiens errants sont une source majeure d'infection à N. caninum dans les fermes laitières de Sanandaj.

Keywords [French]

  • Neospora caninum
  • Chien
  • Renard
  • le Test IFA
  • la Nested-PCR
  • Sananadaj
  • Iran
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