Assessment of Agaricus Bisporus S-II Extract as a Bio-Controlling Agent against Human Pathogenic Bacterial Species

Document Type : Short Communication

Authors

1 Department of Microbiology, School of Bioengineering and Biosciences, Lovely Professional University, Punjab, India

2 Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Punjab, India

Abstract

Agaricus bisporus mushrooms are well known for their nutritional and medicinal values. A. bisporus is a source of protein (about 40% on a dry basis), ergosterol, several minerals, carbohydrate, and fat. The present study was conducted to investigate the effect of A. bisporus S-II extracts on human pathogenic bacteria in-vitro condition. Totally, three human pathogenic bacterial strains (MTCC culture type) were procured from the Institute of Microbial Technology, India. Out of these three bacterial strains, one was Gram-negative (namely P. aeruginosa MTCC741), and the other two were Gram-positive (B. cereus MTCC9786 and S. aureus MTCC740). Microdilution assay was applied for the evaluation of the minimum inhibitory concentration (MIC). The highest antimicrobial activity was observed in methanol extract (26.5%) against S. aureus MTCC740, compared to ethanol extract (17%). Similar results were obtained for P. aeruginosa MTCC741 (21.8%) and B. cereus MTCC9786 (15%) in methanol extract. Least microbial growth inhibition observed for B. cereus MTCC9786 (13.82%) followed by P. aeruginosa MTCC741 (14%), compared to control in ethanol extract. The highest antimicrobial activity up to 17% with ethanolic extracts recorded against S. aureus MTCC740. The MIC results in microtitre plates showed the growth inhibition of P. aeruginosa MTCC741 and S. aureus MTCC740 at extract concentrations of 15 mg/ml and 20 mg/ml, respectively. However, no MIC detected for B. cereus MTCC9786 below 20 mg/ml extract concentration. Regarding minimum bactericidal concentration, the bactericidal value for P. aeruginosa MTCC741 and S. aureus MTCC740 was obtained at 10 mg/ml concentration. The present study indicated that the extracts of the A. bisporus S-II mushrooms had promising antimicrobial activities against the tested organisms.

Keywords

Article Title [French]

Évaluation de l'extrait d'agaricus bisporus S-II dans la lutte biologique contre les espèces bactériennes pathogènes pour l’homme

Abstract [French]

Agaricus bisporus est une espèce de champignons qui sont bien connus pour leurs valeurs nutritionnelles et médicinales. A. bisporus est une source de protéines (environ 40% sur base sèche), d'ergostérol, de plusieurs minéraux, de glucides et de lipides. La présente étude a été menée pour étudier l'effet des extraits d' A. bisporus S-II sur certaines bactéries pathogènes pour l’hommedans des conditions in vitro. Au total, trois souches bactériennes pathogènes (type de culture MTCC) ont été obtenues auprès de l'Institut de technologie microbienne (Inde). Sur ces trois souches bactériennes, une était à Gram négatif (P. aeruginosa MTCC741) et les deux autres étaient Gram positifs (B. cereus MTCC9786 et S. aureus MTCC740). La concentration minimale inhibitrice (CMI) a été évaluée par la méthode de microdilution. L'extrait méthanolique montrait une 'activité antimicrobienne plus élevée contre S. aureus MTCC740 (26.5%), comparé à l'extrait 'éthanolique (17%). Des.résultats similaires ont été obtenus pour P. aeruginosa MTCC741 (21.8%) et B. cereus MTCC9786 (15%) avec une efficacité plus importante des extraits méthanoliques. Pour l'extrait éthanolique une moindre inhibition de la croissance microbienne a été observée pour B. cereus MTCC9786 (13.82%) ainsi que pour P. aeruginosa MTCC741 (14%), par rapport au témoin. L'activité antimicrobienne la plus élevée des extraits éthanoliques a été observée contre S. aureus MTCC740 (17%|) La détermination de la CMI des extraits sur des plaques de microtitration montre une inhibition de la croissance de P. aeruginosa MTCC741 et S. aureus MTCC740 à des concentrations d'extrait de 15 mg/ml et 20 mg/ml, respectivement. Cependant, aucune CMI n'a été observée pour B. cereus MTCC9786 en dessous de la concentration d'extrait de 20 mg/ml. Concernant la concentration bactéricide minimale, la valeur bactéricide pour P. aeruginosa MTCC741 et S. aureus MTCC740 a été obtenue à une concentration de 10 mg/ml. Cetteétude montre que les extraits des champignons A. bisporus S-II présentent une activité antimicrobienne prometteuse contre les organismes testés.

Keywords [French]

  • Antibactérien
  • Bacillus cereus
  • Champignon bouton
  • Pathogènes humains
  • Extrait de champignon

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Archives of Razi Institute
Volume 75, Issue 1
March 2020
Pages 123-130

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