Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats

Document Type: Original Articles


1 Department of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran


This study aimed at investigating the effects of intraperitoneal (IP) administration of magnesium sulfate (MgSO4) on testicular ischemia-reperfusion (IR) injury in rats. In total, 50 adult Wistar rats were randomly divided into 5 groups. Group 1 received no injection (control); however, group 2 was subjected to 2 h of I and 24 h of R. Subsequently, group 3 was subjected to 2 h of 1, and after 1 h of I, 125 mg/kg MgSO4 was injected intraperitoneally followed by 24 h of R. Groups 4 and 5 were subjected to the same process as group 3, whereas the rats were injected with 250 and 500 mg/kg of MgSO4, respectively. After 24 h, the left testes of all rats were removed for histological analysis and antioxidant activities. According to the results, there was a significant increase in tissue malondialdehyde (MDA) among I/R rats (P<0.05), whereas MgSO4 decreased I/R-induced MDA (P<0.05). Furthermore, experimental I/R diminished glutathione peroxidase (GPx) and superoxide dismutase (SOD) levels significantly (P<0.05). Moreover, MgSO4 (250 and 500 mg/kg) increased GPx and SOD activity significantly in I/R rats (P<0.05). Furthermore, seminiferous tubules degenerated, and few spermatocytes were observed in the testis tubules of the I/R rats. Regarding pathological parameters, seminiferous tubules and spermatocyte were normal in the testes of MgSO4 (250 and 500 mg/kg)-treated experimental I/R-induced rats. In conclusion, this study demonstrated the beneficial effects of MgSO4 on testicular IR injury in rats. 


Article Title [French]

Les Effets de l'Administration de Sulfate de Magnésium sur l'Ischémie Testiculaire/Lésion de Reperfusion chez Le Rat

Abstract [French]

Cette étude visait à étudier les effets de l'administration intrapéritonéale (IP) de sulfate de magnésium (MgSO4) sur les lésions d'ischémie-reperfusion (IR) testiculaire chez le rat. Au total, 50 rats Wistar adultes ont été répartis au hasard dans 5 groupes. Le groupe 1 n'a reçu aucune injection (témoin); alors que le groupe 2 a été soumis à 2 h de I et 24 h de R.Ensuite, le groupe 3 a été soumis à 2 h de I suivi d’une heure de I. Un total de125 mg/kg de MgSO4 ont été injectés par voie intrapéritonéale, suivis de 24 h de R. Les groupes 4 et 5 ont été soumis au même protocole que le groupe 3, à la différence que les rats ont reçu respectivement 250 et 500 mg/kg de MgSO4. Les testicules gauches de tous les rats ont été prélevés pour une analyse histologique et de l’activité antioxydantes après 24 h. Selon nos résultats, il y avait une augmentation significative du malondialdéhyde tissulaire (MDA) chez les rats I/R (P<0.05), tandis que le MgSO4 diminuait la MDA induite par l'I/R (P<0.05). De plus, les I/R expérimentaux ont diminué de manière significative les niveaux de glutathion peroxydase (GPx) et de superoxyde dismutase (SOD) (P<0.05). En outre, le MgSO4 (250 et 500 mg/kg) a augmenté significativement l'activité GPx et SOD chez les rats I/R (P<0.05). De plus, les tubules séminifères ont dégénéré et peu de spermatocytes ont été observés dans les tubules testiculaires des rats I/R. Les tubules séminifères et les spermatocytes étaient normaux concernant les paramètres pathologiques dans les testicules de rats expérimentaux induits par I/R traités par MgSO4 (250 et 500 mg/kg). En conclusion, cette étude a démontré les effets bénéfiques du MgSO4 sur les lésions IR testiculaires chez le rat.

Keywords [French]

  • Ischémie/reperfusion (I/R)
  • Sulfate de magnésium
  • rat
  • Testicule
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