Effects of Magnesium Sulfate Administration on Testicular Ischemia/Reperfusion Injury in Rats

Document Type: Original Articles

Authors

1 Department of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

This study aimed at investigating the effects of intraperitoneal (IP) administration of magnesium sulfate (MgSO4) on testicular ischemia-reperfusion (IR) injury in rats. In total, 50 adult Wistar rats were randomly divided into 5 groups. Group 1 received no injection (control); however, group 2 was subjected to 2 h of I and 24 h of R. Subsequently, group 3 was subjected to 2 h of 1, and after 1 h of I, 125 mg/kg MgSO4 was injected intraperitoneally followed by 24 h of R. Groups 4 and 5 were subjected to the same process as group 3, whereas the rats were injected with 250 and 500 mg/kg of MgSO4, respectively. After 24 h, the left testes of all rats were removed for histological analysis and antioxidant activities. According to the results, there was a significant increase in tissue malondialdehyde (MDA) among I/R rats (P<0.05), whereas MgSO4 decreased I/R-induced MDA (P<0.05). Furthermore, experimental I/R diminished glutathione peroxidase (GPx) and superoxide dismutase (SOD) levels significantly (P<0.05). Moreover, MgSO4 (250 and 500 mg/kg) increased GPx and SOD activity significantly in I/R rats (P<0.05). Furthermore, seminiferous tubules degenerated, and few spermatocytes were observed in the testis tubules of the I/R rats. Regarding pathological parameters, seminiferous tubules and spermatocyte were normal in the testes of MgSO4 (250 and 500 mg/kg)-treated experimental I/R-induced rats. In conclusion, this study demonstrated the beneficial effects of MgSO4 on testicular IR injury in rats. 

Keywords


Article Title [French]

Les Effets de l'Administration de Sulfate de Magnésium sur l'Ischémie Testiculaire/Lésion de Reperfusion chez Le Rat

Abstract [French]

Cette étude visait à étudier les effets de l'administration intrapéritonéale (IP) de sulfate de magnésium (MgSO4) sur les lésions d'ischémie-reperfusion (IR) testiculaire chez le rat. Au total, 50 rats Wistar adultes ont été répartis au hasard dans 5 groupes. Le groupe 1 n'a reçu aucune injection (témoin); alors que le groupe 2 a été soumis à 2 h de I et 24 h de R.Ensuite, le groupe 3 a été soumis à 2 h de I suivi d’une heure de I. Un total de125 mg/kg de MgSO4 ont été injectés par voie intrapéritonéale, suivis de 24 h de R. Les groupes 4 et 5 ont été soumis au même protocole que le groupe 3, à la différence que les rats ont reçu respectivement 250 et 500 mg/kg de MgSO4. Les testicules gauches de tous les rats ont été prélevés pour une analyse histologique et de l’activité antioxydantes après 24 h. Selon nos résultats, il y avait une augmentation significative du malondialdéhyde tissulaire (MDA) chez les rats I/R (P<0.05), tandis que le MgSO4 diminuait la MDA induite par l'I/R (P<0.05). De plus, les I/R expérimentaux ont diminué de manière significative les niveaux de glutathion peroxydase (GPx) et de superoxyde dismutase (SOD) (P<0.05). En outre, le MgSO4 (250 et 500 mg/kg) a augmenté significativement l'activité GPx et SOD chez les rats I/R (P<0.05). De plus, les tubules séminifères ont dégénéré et peu de spermatocytes ont été observés dans les tubules testiculaires des rats I/R. Les tubules séminifères et les spermatocytes étaient normaux concernant les paramètres pathologiques dans les testicules de rats expérimentaux induits par I/R traités par MgSO4 (250 et 500 mg/kg). En conclusion, cette étude a démontré les effets bénéfiques du MgSO4 sur les lésions IR testiculaires chez le rat.

Keywords [French]

  • Ischémie/reperfusion (I/R)
  • Sulfate de magnésium
  • rat
  • Testicule
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