Aflatoxin M1-Binding Ability of Selected Lactic Acid Bacteria Strains and Saccharomyces boulardii in the Experimentally Contaminated Milk Treated with Some Biophysical Factors

Document Type: Original Articles

Authors

1 Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Food and Drug Laboratory Research Center, Food and Drug Organization, Ministry of Health and Medical Education, Tehran, Iran

Abstract

There is a growing concern regarding the recurrent observation of aflatoxins (AFs) in the milk of lactating animals. Regarding this, the present study was conducted to assess the aflatoxin M1 (AFM1)-binding ability of three species, namely Lactobacillus rhamnosus, L. plantarum, and Saccharomyces boulardii, inAFM1-contaminatedmilk. The mentioned species were administeredatthe concentrations of107 and 109 CFU/mLto skimmed milk contaminated with 0.5 and 0.75 ng/mL AFM1 within the incubation times of 30 and 90 min at 4°C and 37°C. Lactobacillus rhamnosus was found to have the best binding ability at the concentrations of 107 and 109 (CFU/ml), rendering 82% and 90% removal in the milk samples with 0.5 and 0.75 ng/ml AFM1, respectively. Accordingly, this value at 107 and 109 CFU/ml of L. plantarum was obtained 89% and 82% with 0.75 ng/ml of AFM1, respectively. For S. boulardii at 107 and 109 CFU/ml, the rates were respectively estimated at 75% and 90% with 0.75 ng/ml of AFM1. The best AFM1-binding levels for L. rhamnosus, L. plantarum, and S. boulardii were 91.82±10.9%, 89.33±0.58%, and 93.20±10.9, respectively, at the concentrations of 1×109, 1×107, and 1×107 CFU/ml at 37, 4, and 37°C, respectively. In this study, the maximum AFM1 binding (100.0±0.58) occurred while a combination of the aforementioned probiotics was employed at a concentration of 1×107 CFU/ml at 37°C with 0.5 ng/ml AFM1, followed by the combination of L. rhamnosus and L. plantarum (95.86±10.9) at a concentration of 1×109 CFU/ml at the same temperature with 0.75 ng/ml AFM1. It was concluded that the use of S. boulardii in combination with Lactobacillus rhamnosus and L. plantarum, which bind AFM1 in milk, can decrease the risk of AFM1 in dairy products.

Keywords


Article Title [French]

Capacité de Liaison À l'aflatoxine M1 présente dans le Lait Contaminé de Certaines Souches de Bactéries Lactiques Sélectionnées et de Saccharomyces Boulardii Expérimentalement Traité avec Certains Facteurs Biophysiques

Abstract [French]

Il existe une préoccupation croissante concernant l'observation récurrente des aflatoxines (AF) dans le lait des animaux en lactation. L’obejectif de cette étude était d’évaluer la capacité de liaison de l'aflatoxine M1 (AFM1) de trois espèces, à savoir Lactobacillus rhamnosus, L. plantarum et Saccharomyces boulardii, dans le lait contaminé par FM1. Les espèces ont été administrées à des concentrations de 107 et 109 UFC/ mL dans du lait écrémé contaminé par 0.5 et 0.75 ng/mL d'AFM1 dans des temps d'incubation de 30 et 90 min à 4° C et 37° C. Lactobacillus rhamnosus s'est avéré avoir la meilleure capacité de liaison aux concentrations de 107 et 109 (UFC/ml). Cette bactérie lactique arespectivement éliminé 82% et 90% de l’AFM1 présente dans les échantillons de lait contenant 0.5 et 0.75 ng/ml d’aflatoxines. L. plantarum ajouté au lait à concentration de 107 et 109 UFC/ml s’est également avérée efficace et a diminué le contenu en AFM1 (0.75 ng/ml) de 89% et 82%, respectivement. Pour S. boulardii à 107 et 109 UFC/ml, les taux ont été respectivement estimés à 75% et 90% avec 0.75 ng/ml dAFM1. Les meilleurs niveaux de liaison à l'AFM1 ont été obtenus pour 1×109 UFC/ml de L. rhamnosus, 1×107 UFC/ml de L. plantarum et 1×107 UFC/ml de S. boulardii éliminant respectivement 91.82±10.9%, 89.33±0.58% et 93.20±10.9 dAFM1à 37, 4 et 37 C. Dans cette étude, la liaison maximale à l'AFM1 (100.0±0.58%) s'est produite alors qu'une combinaison des probiotiques susmentionnés était utilisée à une concentration de 1×107 CFU/ml à 37°C avec 0.5 ng/ml d'AFM1. lL combinaison de L. rhamnosus et L. plantarum à une concentration de 1×109 UFC/ml à la même température avec 0.75 ng/ml d'AFM1s’est également montrée hautement efficace (95.86±10.9%). L'utilisation de S. boulardii en combinaison avec Lactobacillus rhamnosus et L. plantarum, peut considérablement réduire le risque de contamination a l’AFM1 dans les produits laitiers.

Keywords [French]

  • AFM1
  • Lait
  • Décontamination
  • Saccharomyces boulardii
  • lactobacillus rhamnosus
  • Lactobacillus plantarum
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