Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in Iran

Document Type: Original Articles

Authors

1 Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran

2 Agricultural Research, Education and Extension Organization (AREEO), Lorestan Agricultural and Natural Resources Research Center, Khorramabad, Iran

3 Department of Medical Microbiology, Lorestan University of Medical Sciences, Khorramabad, Iran

4 Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

5 High Education Institute of Rahyan Novin Danesh (RND), Sari, Iran

6 Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran

7 Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran

8 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran

9 Department of National Program of Zoonotic Disease, School of Health Management and Information Sciences, Iran University of Medical Sciences, Tehran, Iran

Abstract

The present study was conducted as the first molecular detection of Anaplasma species in tick samples based on the sequencing of major surface proteins 4 (msp4) gene fragments in different parts of Iran. A total of 130 tick specimens were collected from Hormozgan, Lorestan, and Guilan, Iran, within 2015 to 2017. Hyalomma asiaticum, Hyalomma dromedarii, Rhipicephalus sanguineus, and Rhipicephalus (Boophilus) species were identified in different geographical regions. An amplicon of 464-bp msp4 of Anaplasma was amplified using polymerase chain reaction in various tick species. Three sequences, including one Anaplasma marginale from R. (Boophilus) species and two Anaplasma ovis from Rhipicephalus sanguineus, were obtained after sequencing. It is concluded that bovine and ovine anaplasmosis agents are present in tick samples in Iran. The use of the gene families of six major surface proteins for the detection of various Anaplasma species is recommended.

Keywords


Article Title [French]

Détection Moléculaire des Espèces Anaplasma marginale et Anaplasma ovis (Rickettsiales: Anaplasmataceae) Chez les Tiques Ixodidés en Iran

Abstract [French]

: Le but de cette étude était la mise en place d’une méthode de détection moléculaire basée sur le séquençage du gène msp4 des espèces d'Anaplasma dans des échantillons de tiques collectés dans différentes régions d'Iran. Au total, 130 échantillons de tiques ont été collectés dans les provinces de Hormozgan, Lorestan et Guilan de 2015 à 2017. Hyalomma asiaticum, H. dromedarii, Rhipicephalus sanguineus et R. (Boophilus) sp. ont été identifiés dans différentes régions géographiques. Un amplicon de msp4 de 464 pb d'Anaplasma de diverses espèces a été amplifié par PCR. Trois séquences, dont une appartenant à Anaplasma marginale de R. (Boophilus) sp. et deux à A. ovis de Rhipicephalus sanguineus ont été obtenus après séquençage. Ces résultats montrent que les anaplasmoses bovines et ovines peuvent potentiellement être transmises par des tiques en Iran. Cette étude suggère également que l’utilisation des familles de gènes de six protéines de surface majeures est efficace dans la détection de diverses espèces d'Anaplasma.

Keywords [French]

  • Anaplasma
  • Arbre phylogénétique
  • msp4
  • Iran
  • Tique
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