Subcutaneous Hydatid Cyst in Laboratory Mice: Is it a Suitable Method for Evaluating Therapeutic Agents against Hydatid Cyst?

Document Type: Original Articles

Authors

1 Department of Parasitology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran

2 Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

Abstract

Hydatid disease is an economic and public health concern in many countries. Currently, surgery is the main treatment option for hydatid disease. In the surgical treatment of hydatidosis, the use of scolicidal agents is very important due to inactivating live protoscoleces and preventing the recurrence of infection. Therefore, it is necessary to investigate newscolicidal agents and novel medications with higher safety and efficacy. In the previous in vitro studies, the scolicidal effects of the methanolic extracts and aromatic water of Zataria multiflora (Z. multiflora) have been demonstrated. Consequently, in this study, the impact of the nanoemulsion of Z. multiflora essential oil on subcutaneous hydatid cysts was compared with albendazole (ABZ). Fifty laboratory male mice were inoculated with 300 viable protoscoleces subcutaneously on the two sides of the abdomen. Following five months of infection, the remaining infected mice (n=42) were allocated into two treatment and one control (without treatment) groups containing fourteen animals each. Group A received ABZ at the dose of 50 mg/kg for 60 days, group B received the nanoemulsions of Z. multiflora at the dose of 50 mg/kg in drinking water for 60 days, and group C was considered as the control group. All the infected mice were euthanized and necropsied two months post-intervention. Afterwards, the cysts were cautiously collected and their number, size, and weight were compared between the mice of different groups. The mean number of hydatid cysts indicated that the nanoemulsion of Z. multiflora essence had a relative superiority to ABZ. On the other hand, the therapeutic effect of ABZ was higher than the nanoemulsion of Z. multiflora essential oil in terms of the mean weight and mean size of hydatid cysts. However, no significant difference was observed between the groups (P>0.5). Overall, the number, weight, and size of cysts were not significantly different between the groups in this investigation. The lack of satisfactory therapeutic results in this study might be due to the location of hydatid cysts in the subcutaneous space.

Keywords


Article Title [French]

Kyste Hydatique Sous-Cutané chez les Souris de Laboratoire: Existe-t-il une Méthode Appropriée pour Évaluer les Agents Thérapeutiques Contre le Kyste Hydatique?

Abstract [French]

L'hydatidose est un problème sanitaire et économique grave dans de nombreux pays. Actuellement, la chirurgie est le principal traitement de la maladie hydatique. Il est très important d'utiliser des agents scolicides dans le traitement chirurgical de l'hydatidose, en raison de l'inactivation des protoscoléces vivants et de la prévention de la récurrence de l'infection. Par conséquent, il est nécessaire d'étudier de nouveaux agents scolicides ainsi que l’efficacité de nouveaux médicaments plus sûrs et plus efficaces. Des études in vitro antérieures ont montré les effets scolicides des extraits méthanoliques et du distillat aromatique de Zataria multiflora (Z. multiflora). Cette étude a comparé l'impact sur les kystes hydatiques sous-cutanés d’une nano-émulsion d'huile essentielle de Z. multiflora à celui de l'albendazole (ABZ). Au total, 50 souris mâles de laboratoire ont été inoculées avec 300 protoscoléces viables par voie sous-cutanée des deux côtés de l'abdomen.Après cinq mois d'infection, les souris infectées (n=42) ont été réparties en deux groupes de traitement et un groupe témoin (sans traitement) contenant chacun 14 animaux. Le groupe A a reçu ABZ à la dose de 50 mg/kg pendant 60 jours, le groupe B a été traitée avec une nano-émulsion de Z. multiflora à la dose de 50 mg/kg dans l'eau potable pendant 60 jours, et le groupe C a été considéré comme le groupe témoin. Toutes les souris infectées ont été euthanasiées et autopsiées deux mois après l'intervention. Ensuite, les kystes des souris de différents groupes ont été collectés avec précaution et leur nombre, taille et poids ont été comparés. Le nombre moyen de kystes hydatiques a indiqué que la nano-émulsion d'huile essentielle de Z. multiflora avait une efficacité relative supérieure à ABZ. Cependant, l'effet thérapeutique de l'ABZ, en termes de poids moyen et de taille moyenne des kystes hydatiques, était supérieur à celui obtenu pour la nano-émulsion d’huile essentielle de Z. multiflora. Il est à noter qu'aucune différence significative n'a été observée entre les groupes (P>0.5). En général, le nombre, le poids et la taille des kystes n'étaient pas significativement différents entre les différents groupes de cette étude. L'absence de résultats thérapeutiques satisfaisants dans cette étude pourrait être dû à la localisation des kystes hydatiques dans l'espace sous-cutané.

Keywords [French]

  • Albendazole
  • Huile essentielle
  • Kyste hydatique sous-cutané
  • Nanoémulsion
  • Zataria multiflora
Brunetti, E., Kern, P., Vuitton, D.A., 2010. Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Trop 114, 1-16.

Casado, N., Perez-Serrano, J., Denegri, G., Rodriguez-Caabeiro, F., 1996. Development of chemotherapeutic model for the in vitro screening of drugs against Echinoccus granulosus cysts: the effects of an albendazole-albendazole sulphoxide combination. Int J Parasitol 26, 59-65.

Chaudhri, N., Soni, G.C., Prajapati, S., 2015. Nanotechnology: an advance tool for nano-cosmetics preparation. Int J Pharma Res Rev 4, 28-40.

Daniel-Mwuambete, K., Ponce-Gordo, F., Torrado, J., Torrado, S., Cuesta-Bandera, C., 2003. Effect of two formulations of benzimidazole carbamates on the viability of cysts of Echinococcus granulosus in vivo. Parasite 10, 371-373.

El-On, J., 2003. Benzimidazole treatment of cystic echinococcosis. Acta Trop 85, 243-252.

Elissondo, M.C., Albani, C.M., Gende, L., Eguaras, M., Denegri, G., 2008. Efficacy of thymol against Echinococcus granulosus protoscoleces. Parasitol Int 57, 185-190.

Ghosh, V., Mukherjee, A., Chandrasekaran, N., 2013. Formulation and characterization of plant essential oil based nanoemulsion: evaluation of its larvicidal activity against Aedes aegypti. Asian J Chem 25, S321- S323.

Ito, A., 2017. Review of "Echinococcus and Echinococcosis, Part A." edited by R. C. Andrew Thompson, Alan J. Lymbery and Peter Deplazes. Parasit Vectors 10, 408.

Junghanss, T., da Silva, A.M., Horton, J., Chiodini, P.L., Brunetti, E., 2008. Clinical management of cystic echinococcosis: state of the art, problems, and perspectives. Am J Trop Med Hyg 79, 301-311.

Kayaalp, C., Bostanci, B., Yol, S., Akoglu, M., 2003. Distribution of hydatid cysts into the liver with reference to cystobiliary communications and cavity-related complications. Am J Surg 185, 175-179.

Khosravi, A., Franco, M., Shokri, H., Yahya, R.R., 2007. Evaluation of the effects of Zataria multiflora, Geranium pelargonium, Myrth and Lemon essences on immune system function in experimental animals. J Vet Res 62, 119-123.

Laura, C., Celina, E., Sergio, S.B., Guillermo, D., Carlos, L., Luis, A., 2015. Combined flubendazole-nitazoxanide treatment of cystic echinococcosis: Pharmacokinetic and efficacy assessment in mice. Acta Trop 148, 89-96.

Mahboubi, M., Ghazian Bidgoli, F., 2010. In vitro synergistic efficacy of combination of amphotericin B with Myrtus communis essential oil against clinical isolates of Candida albicans. Phytomedicine 17, 771-774.

Moazeni, M., Borji, H., Saboor Darbandi, M., Saharkhiz, M.J., 2017. In vitro and in vivo antihydatid activity of a nano emulsion of Zataria multiflora essential oil. Res Vet Sci 114, 308-312.

Moazeni, M., Larki, S., Saharkhiz, M.J., Oryan, A., Ansary Lari, M., Mootabi Alavi, A., 2014a. In vivo study of the efficacy of the aromatic water of Zataria multiflora on hydatid cysts. Antimicrob Agents Chemother 58, 6003-6008.

Moazeni, M., Larki, S., Pirmoradi, G., Rahdar, M., 2015. Scolicidal effect of the aromatic water of Zataria multiflora: an in vitro study. Comp Clin Pathol 24, 1057-1062.

Moazeni, M., Larki, S., Oryan, A., Saharkhiz, M.J., 2014b. Preventive and therapeutic effects of Zataria multiflora methanolic extract on hydatid cyst: an in vivo study. Vet Parasitol 205, 107-112.

Moazeni, M., Roozitalab, A., 2012. High scolicidal effect of Zataria multiflora on protoccoleces of hydatid cyst: an in vitro study. Comp Clin Pathol 21, 99-104.

Moro, P., Schantz, P.M., 2009. Echinococcosis: a review. Int J Infect Dis 13, 125-133.

Odriozola-Serrano, I., Oms-Oliu, G., Martin-Belloso, O., 2014. Nanoemulsion-based delivery systems to improve functionality of lipophilic components. Front Nutr 1, 24.

Ostertag, F., Weiss, J., McClements, D.J., 2012. Low-energy formation of edible nanoemulsions: factors influencing droplet size produced by emulsion phase inversion. J Colloid Interface Sci 388, 95-102.

Otero-Abad, B., Torgerson, P.R., 2013. A systematic review of the epidemiology of echinococcosis in domestic and wild animals. PLoS Negl Trop Dis 7, e2249.

Rai, M., Kon, K., 2015. Nanotechnology in diagnosis, treatment and prophylaxis of infectious diseases, Academic Press, Massachusetts, USA.

Saei-Dehkordi, S.S., Tajik, H., Moradi, M., Khalighi-Sigaroodi, F., 2010. Chemical composition of essential oils in Zataria multiflora Boiss. from different parts of Iran and their radical scavenging and antimicrobial activity. Food Chem Toxicol 48, 1562-1567.

Sajed, H., Sahebkar, A., Iranshahi, M., 2013. Zataria multiflora Boiss. (Shirazi thyme)--an ancient condiment with modern pharmaceutical uses. J Ethnopharmacol 145, 686-698.

Shokri, H., Asadi, F., Bahonar, A.R., Khosravi, A.R., 2006. The Role of Zataria multiflora Essence (Iranian herb) on Innate Immunity of Animal Model. Iran J Immunol 3, 164-168.

Shokri, H., Sharifzadeh, A., 2015. Zataria multiflora Boiss.: A review study on chemical composition, anti-fungal and anti-mycotoxin activities, and ultrastructural changes. J Herbmed Pharmacol 6, 1-9.