Genetic Affinity of Echinococcus granulosus protoscolex in Human and Sheep in East Azerbaijan, Iran

Document Type: Original Articles

Authors

1 Department of Microbiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Proteomics & Biochemistry section Biotechnology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

3 Department of Parasitology, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Semnan, Iran

4 Department of Parasitology, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Abstract

Echinococcosis caused by the larval form of Echinococcus granulosus (E. granulosus) is known as an important zoonotic disease in various parts of the world, including Iran. The genetic diversity of this parasite is very high, particularly in areas where the disease is endemic. It has been suggested in the literature from different parts of the world that diverse factors, such as parasite life cycle, transmission pathways, pathologic disease, immunization, and disease control can be affected by the genetic diversity of the parasite. Various studies indicated sheep strain G1 as the most common genotype throughout the world. This strain is commonly found in the liver and lung repeatedly causing echinococcosis in humans, sheep, and cattle. The present study was conducted to determine the genetic affinity between the protoscolex of E. granulosus in humans and sheep in East Azerbaijan province, Iran for the first time. A total of 120 hydatid cyst samples were collected, 60 of which were from people who referred to the hospitals of East Azerbaijan and 60 were from the sheep slaughtered in Tabriz slaughterhouse. Following DNA extraction, certain regions of the cox1 gene were amplified and evaluated by the polymerase chain reaction. The replicated parts in all isolates had the same size of 450 bp. Electrophoresis was followed by selecting a total of 60 suitable samples, including 30 human samples and 30 sheep samples and sending them for genome sequencing. The overlap of the samples was investigated using the BLAST software. The results of BLAST, sequencing, and overlap demonstrated a genetic linkage of approximately 91.76% between the protoscolex of E. granulosus in human and sheep.

Keywords


Article Title [French]

Affinité Génétique d'Echinococcus Granulosus Protoscolex chez l'Homme et les Ovins dans l'Est Azerbaïdjan, Iran

Abstract [French]

: L'échinococcose causée par la forme larvaire d'Echinococcus granulosus (E. granulosus) est considérée comme une maladie zoonotique importante dans diverses parties du monde, y compris l'Iran. La diversité génétique de ce parasite est très élevée, en particulier dans les zones où la maladie est endémique. Plusieurs études provenant de différentes parties du monde ont suggéré que la diversité génétique du parasite avait des effets sur le cycle de vie du parasite, les voies de transmission, les maladies pathologiques, l'immunisation et le contrôle des maladies. Diverses études indiquent que la souche ovine G1 est le génotype le plus répandu dans le monde. Cette souche se retrouve couramment dans le foie et les poumons, provoquant à plusieurs reprises l'échinococcose chez l'homme, les ovins et les bovins. L’objectif de cette étude était de déterminer l'affinité génétique pour la première fois entre le protoscolex d'E. granulosus chez l'homme et les ovins dans la province de l'Est Azerbaïdjan (Iran). Un total de 120 échantillons de kystes hydatiques ont été collectés, parmi lesquels 60 échantillons provenaient de personnes référées aux hôpitaux d'Azerbaïdjan de l'Est et les autres (n=60) provenaient d’ovins abattus à l'abattoir de Tabriz. Après l'extraction de l'ADN, certaines régions du gène cox1 ont été amplifiées et évaluées par la réaction en chaîne de la polymérase. Les parties répliquées dans tous les isolats avaient la même taille de 450 pb. L'électrophorèse a été suivie par la sélection d'un total de 60 échantillons, comprenant 30 échantillons humains et 30 échantillons d'ovins pour le séquençage du génome. La méthode BLAST a été utilisé pour étudier le chevauchement des échantillons. Les résultats du BLAST, du séquençage et du chevauchement ont démontré une similarité génétique d'environ 91.76% entre le protoscolex d'E. granulosus chez l'homme et les ovins.

Keywords [French]

  • Extraction d'ADN
  • Affinité génétique
  • Kyste hydatique
  • PCR
  • Séquençage
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