Bradykinin-Potentiating Factors of Venom from Iranian Medically Important Scorpions

Document Type: Original Articles

Authors

1 Central Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

2 Department of Human Bacterial Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

The venom of animals, including snakes, scorpions, and spiders is a complex combination of proteins, peptides, and other biomolecules as well as some minerals. Among the biomolecules of some animal’s venom, small peptides that lack disulfide bands known as Non-Disulfide Bridge Peptides (NDBPs) potentiate the bradykinin by preventing the conversion of angiotensin 1 to angiotensin 2 using the mechanism of inhibiting the Angiotensin-Converting Enzyme activity and finally reducing the blood pressure in the victims. This feature of the NDBPs of animal’s venom is suggested as the potential of biological drugs. This study aimed to isolate venom components of three species of Iranian medically important scorpions and study the bradykinin potentiating effect of them. The scorpion specimens were collected from the venomous animals and antivenom production department of Razi Vaccine and Serum Research Institute, Karaj, Iran. Moreover, venom extraction was performed by electrical shock (5 volts). The obtained liquid venom of three species specimens was frozen and lyophilized immediately and then preserved in a cool and dried place. The isolation of the venom components for each scorpion was carried out using high-performance liquid chromatography. The obtained ranges of venom fractions (zones) were tested on isolated tissues of guinea-pig ileum and rat uterus using organ bath instrumentation in several replicates. The bioassays resulted in the peptides, including Z1 and Z2 regions in the venom fractionsof the Hottentotta saulcyi, Z2 in Odontobuthus doriae, as well as Z2 and Z3 in Mesobuthus eupeus demonstrated bradykinin potentiating effect. It is concluded that Bradykinin Potentiating Factors were traceable in the venom of all three scorpion species. Therefore, these venoms have the therapeutic potential to exploit biological-based drugs.

Keywords

Main Subjects


Article Title [French]

Facteurs Potentialisant la Bradykinine du Venin des Scorpions Médicalement Importants en Iran

Abstract [French]

Le venin des animaux, y compris les serpents, les scorpions et les araignées, est une combinaison complexe de protéines, de peptides et d'autres biomolécules ainsi que de certains minéraux. Parmi les biomolécules du venin de certains animaux, de petits peptides dépourvus de bandes disulfures, appelés peptides de pont non disulfure (NDBP) potentialisent la bradykinine en empêchant la conversion de l'angiotensine 1 en angiotensine 2 avec le mécanisme d'inhibition de l'activité de l'enzyme de conversion de l'angiotensine (ACE) et finalement en réduisant la pression artérielle des victimes. Cette caractéristique des NDBPs de venin chez l'animal les suggère comme le potentiel des médicaments biologiques. L'objectif de la présente étude est d'isoler les composants du venin des trois espèces de scorpions médicalement importants en Iran et d'en étudier l'effet de potentialisation de la bradykinine. Les spécimens de scorpion ont été préparés à partir d'animaux venimeux et du département de production l'antivenin de l'Institut de recherche sur le vaccin et le sérum de Razi (RVSRI), et l'extraction du venin a été réalisée par choc électrique (5 volts). Le venin liquide obtenu de trois spécimens d'espèce a été immédiatement congelé et lyophilisé, puis conservé dans un endroit frais et séché. L'isolation des composants du venin de chaque scorpion a été réalisée par chromatographie liquide à haute performance (HPLC). Les gammes obtenues de fractions de venin (zones) ont été testées sur des tissus isolés de l'iléon de cochon d'Inde et de l'utérus de rat à l'aide d'un instrument à bain d'organe en plusieurs répétitions. Les essais biologiques ont abouti aux peptides comprenant les régions Z1 et Z2 des fractions de venin de Hottentotta sulcyi, Z2 pour Odontobutus doriae et Z2 et Z3 dans les fractions de venom de Mesobuthus eupeus ont démontré un effet de potentialisation de la bradykinine. Il est conclu que les facteurs de potentialisation de la bradykinine (FBP) étaient traçables dans le venin des trois espèces de scorpions. Ces venins ont donc le potentiel thérapeutique d’exploiter des médicaments à base biologique.

Keywords [French]

  • Médicaments Biologiques
  • Facteurs de potentialisation de la bradykinine
  • hypertension
  • Venin de scorpion
A European project supported through the Seventh Framework Program, 2011. What's in venom? Venomics, (FP7 Health).

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