Efficacy of CpG-ODN Administration Routes on Humoral Responses against Newcastle disease in Broilers

Document Type: Original Articles


1 Department of Poultry Health and Diseases, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Graduated, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran


Un-methylated cytosine-phosphate-guanosine oligodeoxynucleotides (CpG-ODN) has been considered as a powerful vaccine adjuvant and recognition of CpG-ODN by chicken leukocytes promotes their ability to fight against infections. In our study, efficacy of different routes of CpG-ODN application as an adjuvant on immune responses (antibody titer together with leukogram) following vaccination against Newcastle disease (ND) has been evaluated in broiler chickens (Ross-308). The results indicated that routes of CpG-ODN administration influence immune responses and comparison effectiveness of CpG-OND delivery routes showed that group vaccinated by eye-drop application had the highest antibody titer than that of the group injected intramuscularly (im) and the difference was significant (p = 0.04) on day 35 of age. Antibody titer of the group treated with Clone 30 plus CpG-ODN via eye-drop route was higher than that of the group vaccinated with clone 30 alone on days 28 and 35 of age and the difference was significant (p = 0.04). Co-administration of both vaccine and CpG improved outcome of leukogram of the chickens on days 21 to 42 of age and among the treated groups, WBC of the group received both vaccine and CpG by eye-drop route significantly (p < 0.05) differed from that of the group vaccinated with clone 30 alone on days 28 and 35 but not on day 42 of age. Average final body weight of the control group did not significantly differ from those of the treated groups at end of the experiment. In conclusion, co-administration of ND vaccine plus CpG-ODN via eye-drop route improves immune responses. 


Main Subjects

Article Title [French]

Efficacité des Voies d'Administration ODN-CpG sur les Réponses Humorales contre la Maladie de Newcastle chez les Poulets de Chair

Abstract [French]

Les oligodésoxynucléotides non méthylés de cytosine-phosphate-guanosine (ODN-CpG) ont été considérés comme un puissant adjuvant vaccinal et la reconnaissance de l'ODN-CpG par les leucocytes du poulet favorise leur capacité à lutter contre les infections. Dans notre étude, l'efficacité des différentes voies d'application de l'ODN-CpG en tant qu'adjuvant des réponses immunitaires (évaluation du titre d'anticorps ainsi que du leucogramme) à la suite de la vaccination contre la maladie de Newcastle (ND) a été étudiée chez des poulets de chair (Ross-308). Les résultats montraient que les voies d'administration de l'ODN-CpG influent sur les réponses immunitaires. Le groupe vacciné par l'application de gouttes oculaires avait un titre d’anticorps plus élevé que celui traité pat injection intramusculaire (im) et la différence était significative (p = 0,04) au trente-cinquième jour. Le titre en anticorps du groupe traité par les gouttes oculaires comprenant le clone 30 combiné à l'ODN-CpG était supérieur aux jours 28 et 35 à celui du groupe vacciné uniquement avec le clone 30 (p = 0,04). La co-administration du vaccin et du CpG a amélioré les résultats du leucogramme des poulets du 21ème au 42ème jour. Parmi les groupes traités, le leucogramme du groupe ayant reçu à la fois le vaccin et le CpG par voie oculaire montrait des différences significatives (p <0,05) de celui du groupe vacciné uniquement avec le clone 30 aux 28ème et 35ème jours, mais au 42ème jour. Le poids corporel final moyen du groupe témoin ne différait pas significativement de celui des groupes traités à la fin de l'expérience. En conclusion, la co-administration du vaccin ND et de l'ODN-CpG améliore la réponse immunitaire après vaccination.

Keywords [French]

  • CpG
  • titre en anticorps
  • ND
  • poulets de chair
  • voie d'administration du vaccin
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