Investigation of the Effects of Spinal Dexamethasone Injection as a Premedication in Rabbit Anesthesia

Document Type : Original Articles


1 Department of Clinical Science, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Pathobiology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran


Anesthesia and analgesia are important in human and veterinary medicine, especially in surgical procedures. Rodents, avians, and exotic species are required to be anesthetized using an appropriate anesthetic regimen. This study aimed to suggest a new anesthetic drug and method in order to facilitate anesthesia as well as analgesia among rabbits, laboratory animals, and humans. Spinal injection of dexamethasone combined with intramuscular ketamine among rabbits can play the role of premedication agents. A total of 24 healthy white adult rabbits from New-Zealand were equally assigned into four groups. Groups 1, 2, 3, and 4 were subjected to spinal xylazine (5mg/kg) with ketamine (35mg/kg,IM), spinal dexamethasone (0.37mg/kg-four times diluted) with ketamine (35mg/kg,IM), dexamethasone (4mg/kg,IM) with ketamine (35mg/kg,IM), and spinal dexamethasone (0.37mg/kg-four times diluted), respectively. The results showed that there was a significant difference in terms of clinical reflexes recorded for group 2, compared to groups 1 and 3. A significant difference was also observed regarding clinical reflexes between group 2 and the other groups. Furthermore, no abnormality was observed in terms of histological sections within groups 2 and 4. Spinal dexamethasone can be used as a premedication combined with ketamine in rabbit anesthesia.


Main Subjects

Article Title [French]

Étude des effets de l'injection spinale de dexaméthasone en tant que prémédication lors de l'anesthésie chez le lapin

Abstract [French]

L'anesthésie et l'analgésie sont importantes en médecine humaine et vétérinaire, en particulier dans les procédures chirurgicales. Les rongeurs, les oiseaux et les espèces exotiques doivent être anesthésiés à l'aide d'un traitement anesthésique approprié. Cette étude visait à suggérer un nouveau médicament anesthésique et une nouvelle méthode afin de faciliter l'anesthésie ainsi que l'analgésie chez les lapins, les animaux de laboratoire et les humains. L'injection spinale de dexaméthasone associée à de la kétamine par voie intramusculaire chez le lapin peut ȇtre ulisée comme prémédication. Au total, 24 lapins blancs adultes de Nouvelle-Zélande, en bonne santé, ont été également répartis dans quatre groupes. Les groupes 1, 2, 3 et 4 ont été soumis à une xylazine spinale (5 mg/kg) avec de la kétamine (35mg/kg, IM), de la dexaméthasone spinale (0.37mg/kg diluée quatre fois) accompagnée de kétamine (35mg/kg,IM), dexaméthasone (4mg/kg,IM) et kétamine (35mg/kg,IM) et la dexaméthasone spinale (0.37mg/kg, diluée quatre fois). Les résultats ont montré qu'il existait une différence significative en termes de réflexes cliniques enregistrés pour le groupe 2 par rapport aux groupes 1 et 3. Une différence significative a également été observée concernant les réflexes cliniques entre le groupe 2 et tous les autres groupes. De plus, aucune anomalie n'a été observée au niveau des coupes histologiques dans les groupes 2 et 4. La dexaméthasone spinale peut donc être utilisée comme prémédication en association avec de la kétamine lors d'une anesthésie chez le lapin.

Keywords [French]

  • Anesthésie
  • Dexaméthasone
  • Prémédication
  • Lapin
  • Colonne vertébrale
Abram, S.E., 2000. Neural Blockade for Neuropathic Pain. Clin J Pain 16, 56-61.
Allen, K., 2007. Dexamethasone: An All Purpose Agent. Aust Anaesth 65-70.
Armand, S., Langlade, A., Boutros, A., 1988. Meta-Analysis of the Efficacy of Extradural Clonidine to Relieve Postoperative Pain: An Impossible Task. Br J Aneasth 81, 126-134.
Bani-Hashem, N., Hassan-Nasab, B., Alijan-Pour, E., AmriMaleh, P., Nabavi, A., Jabbari, A., 2011. Addition of Intrathecal Dexamethasone to Bupivacaine for Spinal Anesthesia in Orthopedic Surgery. Saudi J Anaesth 5, 382–386.
Barros, G., Marques, M., Ganem, E., 2007. The Effects of Intrathecal Administration of Betamethasone over the Dogs' Spinal Cord and Meninges. Acta Cir Bras 22, 361-365.
Bisgaard, T., Klarskov, B., Kehlet, H., Rosenburg, J., 2003. Pre operativedexamethasone Improves Surgical Outcome After Laproscopic Cholecystectomy:A Randomized, Double-Blind Placebo-Controlled Trial. Ann Surgery 238, 651-660.
Cicala, R., Turner, Moran, E., Henley, R., Wong, R., Evans, J., 1990. Methylprednisolone acetate does not cause inflammatory changes in the epidural space. Anesthesiology 72, 556-558.
Di, S., Maxson, M., Franco, A., Tasker, J., 2009. Glucocorticoids Regulate Glutamate and GABA Synapse-Specific Retrograde Transmission via Divergent Nongenomic Signaling Pathways. J Neurosci 29, 393-401.
Fang, H., Zhang, M., Yue, G., 2015. Dexamethasone-Soaked Gelatin Sponges Prevent Epidural Adhesion. J Clin Rehabil Tissue Engin Res 8, 1165-1169.
Hall, L., Clarke, K., Trim, C., 2001. General Principles of Local Analgesia. In: Veterinary Anesthesia, WB Saunders Co.
Hayashi, N., Weinstein, J., Meller, S., Lee, H., Spratt, K., Gebhart, G., 1998. The Effect of Epidural Injection of Betamethasone or Bupivacaine in a Rat Model of Lumbar Radiculopathy. Spine 23, 877-885.
Hedenbro, J., Olsson, A., 1998. Metoclopramide and Ureteric Colic. Acta Chir Scand 157, 439-440
Henzi, I., Walder, B., Tramer, M., 2000. Dexamethasone for the Prevention of Postoperative Nausea Dn Vomiting: A Quantitative Systematic Review. Anesth Analg 90, 186-194.
Ho, C., Ho, S., Wang, J., Tsai, S., Chai, C., 2011. Dexamethasone Prevents Postoperative Nausea And Vomiting. Benefit Risk 49, 100-104.
Ho, C., Wu, H., Ho, S., Wang, J., 2004. Dexamethasone Has a Central Antiemetic Mechanism in Decerebrated Cats. Anesth Analg 99, 734–739.
Jno-Baptiste, B., Scarlett, M.D., Harding, H.E., 2014. The Effect of Dexamethasone on Post-Operative Opioid Requirement in Patients Who Underwent Gynecology Surgery at the University Hospital in Jamaica. J Anesth Clin Res 1, 5-11.
Johnston, M., 2005. Clinical Approaches to Analgesia in Ferrets and Rabbits in Proceeding. Seminars In Avian And Exotic Pet Med 14, 229-235.
Kim, E.M., Lee, J.R., Koo, B.N., Im, Y.J., Oh, H.J., J.H.L., 2014. Analgesic Efficacy Of Caudal Dexamethasone Combined With Ropivacaine In Children Undergoing Orchiopexy. Br. J Anaesth. 112, 885-891.
Malinovsky, J.M., Bernard, J.M., Baudrimont, M., 1997. A Chronic Model for Experimental Investigation of Epidural Anaesthesia in the Rabbit. Reganesth and Pain Med. 22, 80-85.
Marjani, M., Tavakoli, A., J.Tavakoli, 2014. Comparison The Analgesic Effects Of Lidocaine, Xylazine And Their Combination Used Into The Epidural Space In Rabbits. I.J.V.S.T. 9, 17-21.
Min, S.H., Soh, J.S., Park, J.Y., Choi, S.U., Lee, H.W., Lee, J.J., et al., 2014. Epidural Dexamethasone Decreased Inflammatory Hyperalgesia And Spinal CPLA2 Expression In A Rat Formalin Test. Yonsei Med J 55, 1631–1639.
Samarkandi, A., Shaikh, M., Ahmad, R., Alammar, A., 2004. Use of Dexamethasone to Reduce Postoperative Vomiting and Pain after Pediatrictonsillectomy Procedures. Saudi Med J 25, 1636-1639.
Stefan, M.T., Spoorenberg, S.M.C., Rijkers, G.T., Grutters, J.C., E.M.W. Van De Garde, Meijvis, S.C.A., et al., 2015. Antipyretic Effect of Dexamethasone in Community-Acquired Pneumonia. Eur Resp J 46, 570-573.
Tavakoli, A., Kazemi-Mehrjerdi, H., 2011. Addition of Metoclopramide or Tramadol on Analgesic Effects of Lidocaine in Epidural Analgesia in Rabbit. I.J.V.S.T. 3, 41-48.
Tian, F., Dou, C., Qi, S., Zhao, L., Chen, B., Yan, H., et al., 2015. Preventive effect of dexamethasone gelatin sponge on the lumbosacral epidural adhesion. Int J Clin Exp Med 8, 5478.
Tranquili, W.J., Thurmon, J.C., Grimm, K.A., 2007. Lumb & Jones’ Veterinary Anesthesia and Analgesia Blackwell Publishing.
Yasukazu, K., Masahiko, M., Namiko, N., Kiyohisa, T., 1993. Differential Effect of Subchronic Dexamethasone Treatment on Serotonin-2 And/3-Adrenergic Receptors in the Rat Cerebral Cortex and Hippocampus. Neurosci Lett 155, 195-198.
Yousef, G.T., Ibrahim, T.H., Khder, A., Ibrahim, M., 2014. Enhancement of Ropivacaine Caudal Analgesia Using Dexamethasone or Magnesium in Children Undergoing Inguinal Hernia Repair. Anesth Essays Res 8, 13-19.