The Effect of Oral Administration of Zinc Oxide Nanoparticles on Quantitative and Qualitative Properties of Arabic Ram Sperm and Some Antioxidant Parameters of Seminal Plasma in the Non-Breeding Season

Document Type : Original Articles

Authors

1 Department of Animal Science, Ramin Agricultural and Natural Resources University of Khuzestan, Ahwaz, Iran

2 Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Khuzestan, Iran

3 Department of Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

Abstract

Zinc is an essential mineral and accepted as a trace element in the animal nutrition and for its role in biological enzymatic pathways. This study aimed to investigate the effect of oral administration of zinc oxide nanoparticles (ZnONPs) on some of the antioxidant parameters of semen plasma, quantitative and qualitative properties of Arabic ram sperm in the non-breeding season. Twelve adult Arabic rams (about 3-5 years old, 70 ±2.1 kg) were randomly assigned to receive one of the three levels of dietary ZnONPs (control; 0, group 1; 40 ppm and group 2; 80 ppm). Results showed that using different levels of ZnONPs increased the activity of superoxide dismutase enzyme (SOD) and total antioxidant capacity (TAC) of semen plasma significantly compared  with the control group (P<0.05). Motility (74.83%), viability (76.90%), semen volume (1.76 ml) and sperm concentration (1418×106/ml) were significantly (P<0.05) higher in ZnONPs supplemented groups compared with the control group. The sperm morphological abnormalities reduced significantly in treated groups (10.46 and 9.07%) compared with the control group (12.66%; P <0.05). Also, the results suggested that 80 ppm level of ZnONPs increased the functionality of sperm membrane (44.38%) compared with other groups (37 and 35.66%, respectively for groups 1 and control) (P<0.05). Based on the results, using 80 ppm level of ZnONPs lead to an improvement in the activity of superoxide dismutase enzyme (48.62 ml) and total antioxidant capacity of semen plasma (111.88 µg/ml) compared with other groups  (P<0.05). In conclusion using 80 ppm level of ZnONPs had a positive effect on the quantitative and qualitative properties of sperm and lead to a significant betterment in the activity of some antioxidant parameters of Arabic ram semen in the non-breeding season.

Keywords

Main Subjects

Article Title [French]

L’effet de administration orale des nanoparticules d’oxyde de zinc sur les propriétés quantitatives et qualitatives du sperme ainsi que sur les paramètres antioxydants du liquide séminal chez le mouton arabe en-dehors de sa saison de reproduction

Abstract [French]

Le zinc est un minéral essentiel étant considéré comme un oligo-élément dans la nutrition animale, étant donné son rôle dans les voies biologiques enzymatiques. L’objectif de cette étude était d’étudier l’effet de l’administration orale des naoparticules d’oxyde de zinc (ZnONPs) sur les paramètres antioxydants du liquide séminal, ainsi que sur les propriétés quantitative et qualitative du sperme du mouton arabe en-dehors de la saison de reproduction. Un total de 12 moutons arabes adultes a été sélectionné de façon aléatoire pour recevoir l’une des 3 concentrations de ZnONPs (control; 0, groupe 1; 40 ppm and groupe 2; 80 ppm). Nos résultats ont démontré l’effet stimulant de l’administration des différents taux de zinc sur l’activité de l’enzyme superoxide dismutase (SOD) ainsi que sur la capacité antioxydante totale (TAC) du liquide séminal, ces paramétres montrant une augmentation significative comparé au groupe control (P<0.05). La motilité (74.83%), la viabilité (76.90%), le volume du liquide séminal (1.76 ml) et la concentration de sperme (1418×106/ml) étaient significativement plus élevés dans les groupes traités avec ZnONPs comparés au groupe control (P<0.05). De plus, les malformations observées au niveau des spermes des groupes traités (10.46 and 9.07%) étaient mois importantes par rapport au groupe control (12.66%; P <0.05). Nos résultats ont également révélé qu’une supplémentation de 80 ppm de ZnONPs augmente de façon significative la fonctionnalité de la membrane des spermes (44.38%) comparés aux groupes 1 (37) et control (35.66%) (P<0.05). De plus, selon nos résultats, une supplémentation en ZnONPs à une concentration de 80 ppm contribue à l’amélioration de l’activité enzymatique de la superoxide dismutase (48.62 ml) ainsi que de la capacité antioxydante totale du liquide séminal comparé (111.88 µg/ml) au groupe control (P<0.05). En conclusion, une supplémentation de 80 ppm de ZnONPs montre un effet positif sur les propriétés quantitatives et qualitatives du sperme et peut mener à une amélioration significative des paramètres antioxydants du liquide séminal chez le mouton arabe en-dehors de sa saison de reproduction.

Keywords [French]

  • Mouton
  • Sperme
  • Superoxyde dismutase
  • Capacité antioxydant totale
  • Nanoparticules d’oxyde de Zinc

References

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Archives of Razi Institute
Volume 73, Issue 2
June 2018
Pages 121-129

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