Effects of Essential Oils Combination on Sporulation of Turkey (Meleagris gallopavo) Eimeria Oocysts

Document Type: Original Articles


1 Department of Poultry Health & Diseases, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran


Avian coccidiosis is the most important parasitic disease in poultry production, which inflicts numerous losses to the industry. The extensive use of anticoccidial drugs leads to parasite resistance and drug residue in poultry products. In the present study, we aimed to investigate the effects of three famous essential oils (EOs) and their combination on inactivation of mixed oocysts of Eimeria adenoides, Eimeria dispersa, Eimeria meleagrimitis, and Eimeria meleagridis. The EOs of Thymus vulgaris, Artemisia sieberi, and Mentha pulegium were prepared.  After inoculation of each turkey with 7×105 sporulated oocysts, fresh unsporulated oocysts were harvested from their feces. To evaluate the sporulation inhibition effect, 5×104 oocysts were used in each treatment. Each EO was used in increasing concentrations. Half maximal inhibitory concentration (IC50) was determined for each EO and they were blended in pairs based on IC50 line. Our results showed that the IC50 values for mentha, artemisia, and thyme were 22.92, 40.5, and 53.42 mg/ml, respectively. According to our results, artemisia and thyme combination has a synergistic effect, whereas the combination of a high concentration of mentha with a low concentration of thyme had an antagonistic effect. During this study, no interactions were observed between mentha and artemisia.


Main Subjects

Article Title [French]

Effets d'une combinaison d'huiles essentielles sur la sporulation des oocystes d’Eimeria chez les dindes (Meleagris gallopavo)

Abstract [French]

La coccidiose aviaire est la maladie parasitaire la plus importante dans l’industrie aviaire infligeant des pertesimportanantes. La forte utilisation de médicaments anticoccidiens entraîne des résistances aux parasites et la persistance des résidus de médicaments dans les produits de volaille. Cetteétude visait à étudier l'effet de trois célèbres huiles essentielles (EO) et leur combinaison sur l'inactivation d'oocystes mixtes d'Eimeria adenoides, Eimeria dispersa, Eimeria meleagrimitis et Eimeria meleagridis. Les EO de Thymus vulgaris, Artemisia sieberi et x ont été extraites. Après inoculation de chaque dinde avec des oocystes sporulés de 7 x 105, des oocystes frais non sporulés ont été récoltés à partir de leurs fèces. Pour évaluer l'effet 'inhibitoire sur la sporulation, un nombre de 5 × 104 oocystes ont été utilisés dans chaque traitement. Chaque OE a été utilisé dans des concentrations croissantes. La concentration inhibitrice demi-maximale (CI50) a été déterminée pour chaque EO ainsi que sur les mélanges de deux huiles essentielles. Les résultats de cette étude ont montré que la CI50 pour l'origan, l'armoise et le thym était respectivement de 22,92, 40,5 et 53,42 mg/ml. Selon nos résultats, l'interaction de l'armoise et du thym conduit à un effet synergique alors que la combinaison d'une forte concentration d'origan et d'une faible concentration de thym a des effets antagonistes. Au cours de cette étude, aucune interaction entre l'origan et l'armoise n'a été observée.

Keywords [French]

  • Huiles essentielles
  • Coccidiose
  • Turquie
  • Eimeria
  • Inhibition de la sporulation
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