Effects of Essential Oils Combination on Sporulation of Turkey (Meleagris gallopavo) Eimeria Oocysts

Document Type : Original Articles

Authors

1 Department of Poultry Health & Diseases, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Abstract

Avian coccidiosis is the most important parasitic disease in poultry production, which inflicts numerous losses to the industry. The extensive use of anticoccidial drugs leads to parasite resistance and drug residue in poultry products. In the present study, we aimed to investigate the effects of three famous essential oils (EOs) and their combination on inactivation of mixed oocysts of Eimeria adenoides, Eimeria dispersa, Eimeria meleagrimitis, and Eimeria meleagridis. The EOs of Thymus vulgaris, Artemisia sieberi, and Mentha pulegium were prepared.  After inoculation of each turkey with 7×105 sporulated oocysts, fresh unsporulated oocysts were harvested from their feces. To evaluate the sporulation inhibition effect, 5×104 oocysts were used in each treatment. Each EO was used in increasing concentrations. Half maximal inhibitory concentration (IC50) was determined for each EO and they were blended in pairs based on IC50 line. Our results showed that the IC50 values for mentha, artemisia, and thyme were 22.92, 40.5, and 53.42 mg/ml, respectively. According to our results, artemisia and thyme combination has a synergistic effect, whereas the combination of a high concentration of mentha with a low concentration of thyme had an antagonistic effect. During this study, no interactions were observed between mentha and artemisia.

Keywords

Main Subjects


Article Title [French]

Effets d'une combinaison d'huiles essentielles sur la sporulation des oocystes d’Eimeria chez les dindes (Meleagris gallopavo)

Abstract [French]

La coccidiose aviaire est la maladie parasitaire la plus importante dans l’industrie aviaire infligeant des pertesimportanantes. La forte utilisation de médicaments anticoccidiens entraîne des résistances aux parasites et la persistance des résidus de médicaments dans les produits de volaille. Cetteétude visait à étudier l'effet de trois célèbres huiles essentielles (EO) et leur combinaison sur l'inactivation d'oocystes mixtes d'Eimeria adenoides, Eimeria dispersa, Eimeria meleagrimitis et Eimeria meleagridis. Les EO de Thymus vulgaris, Artemisia sieberi et x ont été extraites. Après inoculation de chaque dinde avec des oocystes sporulés de 7 x 105, des oocystes frais non sporulés ont été récoltés à partir de leurs fèces. Pour évaluer l'effet 'inhibitoire sur la sporulation, un nombre de 5 × 104 oocystes ont été utilisés dans chaque traitement. Chaque OE a été utilisé dans des concentrations croissantes. La concentration inhibitrice demi-maximale (CI50) a été déterminée pour chaque EO ainsi que sur les mélanges de deux huiles essentielles. Les résultats de cette étude ont montré que la CI50 pour l'origan, l'armoise et le thym était respectivement de 22,92, 40,5 et 53,42 mg/ml. Selon nos résultats, l'interaction de l'armoise et du thym conduit à un effet synergique alors que la combinaison d'une forte concentration d'origan et d'une faible concentration de thym a des effets antagonistes. Au cours de cette étude, aucune interaction entre l'origan et l'armoise n'a été observée.

Keywords [French]

  • Huiles essentielles
  • Coccidiose
  • Turquie
  • Eimeria
  • Inhibition de la sporulation
Allen, P.C., Lydon, J., Danforth, H.D., 1997. Effects of components of Artemisia annua on coccidia infections in chickens. Poult Sci 76, 1156-1163.
Belli, S.I., Smith, N.C., Ferguson, D.J.P., 2006. The coccidian oocyst: a tough nut to crack! Tren Parasitol 22, 416-423.
Bozkurt, M., Giannenas, I., Küçükyilmaz, K., Christaki, E., Florou-Paneri, P., 2013. An update on approaches to controlling coccidia in poultry using botanical extracts. British Poult Sci 54, 713-727.
Brenes, A., Roura, E., 2010. Essential oils in poultry nutrition: Main effects and modes of action. Anim Feed Sci Technol 158, 1-14.
Burt, S., 2004. Essential oils: their antibacterial properties and potential applications in foods--a review. Int J Food Microbiol 94, 223-253.
Conway, D.P., McKenzie, M.E., 2008. Poultry Coccidiosis: Diagnostic and Testing Procedures, 3rd ed., Blackwell Publishing Professional, Iowa (USA),pp. 41-47.
delCacho, E., Gallego, M., Francesch, M., Quilez, J., Sanchez-Acedo, C., 2010. Effect of artemisinin on oocyst wall formation and sporulation during Eimeriatenella infection. ParasitolInt 59, 506-511.
Delaquis, P.J., Stanich, K., Girard, B., Mazza, G., 2002. Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils. Int J Food Microbiol 74, 101-109.
Duru, M.E., Ozturk, M., Ugur, A., Ceylan, O., 2004. The constituents of essential oil and in vitro antimicrobial activity of Micromeriacilicica from Turkey. J Ethnopharmacol 94, 43-48.
Fatemi, A., Razavi, S.M., Asasi, K., TorabiGoudarzi, M., 2015. Effects of Artemisia annua extracts on sporulation of Eimeria oocysts. Parasitol Res 114, 1207-1211.
Gessner, P.K., 1995. Isobolographic analysis of interactions: an update on applications and utility. Toxicol 105, 161-179.
Guimaraes, J.S., Jr., Bogado, A.L., da Cunha, T.C., Garcia, J.L., 2007. In vitro evaluation of the disinfection efficacy on Eimeriatenellaunsporulated oocysts isolated from broilers. Rev BraParasitol Vet 16, 67-71.
Kennedy, D.G., Smyth, W.G., Hewitt, S.A., Mcevoy, J.D., 1998. Monensin carry-over into unmedicated broiler feeds. Analyst 123, 2529-2533.
Mahboubi, M., Valian, M., Kazempour, N., 2015. Chemical composition, antioxidant and antimicrobial activity of Artemisia sieberi oils from different parts of Iran and France. JEssential Oil Res 27, 140-147.
Mcdougald, L.R., Fitz-Coy, S.H., 2013. Coccidiosis. In: Swayne, D.E., Glisson, J.R., Mcdougald, L.R., Nolan, L.K., Suarez, D.L., Nair, V. (Eds.), Diseases of Poultry13th edn.,Wiley-Blackwell, USA, pp. 1163-1165.
Molan, A.L., Liu, Z., De, S., 2009. Effect of pine bark (Pinusradiata) extracts on sporulation of coccidian oocysts. Folia Parasitol (Praha) 56, 1-5.
Muthamilselvan, H., Kuo, T.F., Wu, Y.C., Yang, W.C., 2016. Herbal remedies for coccidiosis control: a review of plants, compounds, and anticoccidial actions.  Evidence-Based Complementary and Alternative Medicine 2016, Article ID 2657981.
Oumzil, H., Ghoulami, S., Rhajaoui, M., Ilidrissi, A., Fkih-Tetouani, S., Faid, M., et al., 2002. Antibacterial and antifungal activity of essential oils of Menthasuaveolens. Phytother Res 16, 727-731.
Peek, H., 2010. Resistance to anticoccidial drugs: alternative strategies to control coccidiosis in broilers. Department of pathobiology Utrecht Faculty Veterinary Medicine, University Utrecht, p. 244.
Remmal, A., Bouchikhi,T., Rhayour, K., Ettayebi, M.,1993. Improved Method for the Determination of Antimicrobial Activity of Essential Oils in Agar Medium. J Essen Oil Res 5, 197-184.
Remmal, A., Achahbar, S., Bouddine, L., Chami, N., Chami, F., 2011. In vitro destruction of Eimeria oocysts by essential oils. Vet Parasitol 182, 121-126.
Saini, R., Davis, S., Dudley-Cash, W., 2003. Mentha essential oil reduces the expression of coccidiosis in broilers. Proceeding of the 52nd Conference on Western Poultry Diseases, Sacramento, CA, pp. 97-98.
Samaha, H.A.t., Haggag, Y.N., Nossair, M.A.s., Habib, H.M., 2013. Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis. AJVS 39, 82-90.
Shirley, M.W., 1992. Research on avian coccidia: An update. Brit Vet J 148, 479-499.
Shivaramaiah, C., John R Barta, Xochitl Hernandez-Velasco, Guillermo Téllez, Billy M Hargis, 2014. Coccidiosis: recent advancements in the immunobiology of Eimeria species, preventive measures, and the importance of vaccination as a control tool against these Apicomplexan parasites. Vet Med 5, 23.
Solórzano-Santos, F., Miranda-Novales, M.G., 2012. Essential oils from aromatic herbs as antimicrobial agents. CurrOpinBiotechnol 23, 136-141.
Stephen, B., Rommel, M., Daugschies, A., Haberkorn, A., 1997. Studies of resistance to anticoccidials in Eimeria field isolates and pure Eimeria strains. Vet Parasitol 69, 19-29.
Vrba,V., Pakandl, M., 2014. Coccidia of turkey: from
isolation, characterisation and comparison to molecular phylogeny and molecular diagnostics. Int J Parasitol 44, 985-1000.
Williams, R.B., 1997. Laboratory tests of phenolic disinfectants as oocysticides against the chicken coccidiumEimeriatenella. Vet Rec 141, 447-448.
Zaman, M.A., Iqbal, Z., Khan, M.N., Muhammad, G., 2012. Anthelmintic activity of a herbal formulation against gastrointestinal nematodes of sheep. Pak Vet J 32, 117-121.
Zhou, F., Ji, B., Zhang, H., Jiang, H., Yang, Z., Li, J., et al., 2007. Synergistic effect of thymol and carvacrol combined with chelators and organic acids against Salmonella Typhimurium. J Food Prot 70, 1704-1709.