Infectious Causes of Bovine Abortion in Qazvin Province, Iran

Document Type : Original Articles

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran

2 Graduated, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran

3 Department of Pathology, Qazvin University of Medical Sciences, Qazvin, Iran

Abstract

Abortion in dairy cattle is generally defined as the loss of fetus on days 47-265 of pregnancy and can significantly reduce the income and capital gain of farmers and producers. Therefore, sufficient economical support and preventive measurements are essential for this issue. In this study, genetic materials were extracted from the abomasum rennet, as well as homogenous preparations of brain, kidney, spleen, liver, and lung tissues of 128 aborted fetuses. Afterward, polymerase chain reaction (PCR) and Reverse Transcription Polymerase Chain Reaction (RT-PCR) tests were performed to identify the bovine viral diarrhea virus (BVDV), bovine herpesvirus 1 (BHV-1), Neospora caninum parasite, and serovars of Leptospira species. In the PCR test, the samples with 380 bp, 340 bp, and 173 bp bands were considered as positive for serovars of Leptospira, Neospora caninum, and BHV-1, respectively. Moreover, a 290 bp band was regarded to be BVDV in the RT-PCR. According to the findings of the current study, 39 (30.47%) of the samples were infected with Neospora caninum, 26 (20.31%) with BVDV, 17 (13.28%) with BHV-1, and 18 (14.06%) with serovars of Leptospira. In addition, multiple-agent infections were also detected in the samples. As a conclusion, the microorganism Neospora caninum was identified as the most prevalent infectious cause of abortion in the eight agro-industrial and livestock complexes, in Qazvin province, Iran.

Keywords

Main Subjects

Article Title [French]

Causes Infectieux d'Avortement bovine en Province Qazvin, Iran

Abstract [French]

L'avortement chez les vaches laitières se réfère généralement à la mort du fœtus entre les jours 47 à 265 de la grossesse. Étant donné que l'avortement peut engendrer la réduction considérable des revenus et la perte de capital des producteurs, il est nécessaire de prendre des mesures de prévention et d'accorder un budget approprié à cet effet. Dans la présente étude, 128 fœtus avortés provenant de 8 fermes laitières de la province de Qazvin en Iran ont été examinés au second semestre de l’an 2015. Les échantillons de fœtus testés comprenaient un mélange homogène des tissus du cerveau, des reins, de la rate, du foie et des poumons du fœtus avorté. Après l'extraction du matériel génétique, des expériences PCR et RT-PCR ont été réalisées pour identifier BVDV, BHV-1, parasite de Neospora caninum et la bactérie de Leptospirose. Finalement, en effectuant le test de PCR, les échantillons de bandes bp380 pour les bactéries leptospires, bandes bp340 pour le parasite de Neospora caninum et la bande bp173 pour le virus BHV-1 ont été considérés comme positifs dans l'électrophorèse sur gel. En effectuant également le test RT-PCR, le résultat de la bande bp290 pour le virus BVDV était positif. À la fin, sur 128 fœtus avortés, 39 échantillons (30,47%) ont été infectés par Neospora caninum, 26 (20,31%) ont été infectés par le virus BVDV, 17 (13,28%) ont été infectés par le BHV- 1 et 18 échantillons (14,06%) ont été infectés par les bactéries de Leptospirose.

Keywords [French]

  • avortement
  • réaction en chaîne par polymérase
  • Neospora caninum
  • Diarrhée virale bovine
  • Herpèsvirus Bovin de Type 1
  • Leptospira

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Archives of Razi Institute
Volume 72, Issue 4
November and December 2017
Pages 225-230

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