Isolation and Detection of Mycoplasma agalactiae from Semen Samples of Goats

Document Type: Original Articles

Authors

1 Mycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

2 Master

3 Assistant Professor

4 Bachelor

Abstract

Contagious agalactia (CA) is a highly infectious disease of goats and sheep, and is a form of Mycoplasmosis,
which is usually enzootic. Since Mycoplasma agalactiae (M. agalactiae) is the main cause of this disease in
goats, the aim of this study was to isolate and detect M. agalactiae from semen of goat bucks. Thirty-nine semen
samples were collected from goat bulks, and all samples were cultured in PPLO broth medium supplemented for
M. agalaciae isolation. The bacteria DNAs were extracted from clinical samples and the PCR assay was applied
to detect Mycoplasma genus and M. agalactiae species using specific primers, which amplified a 163bp
fragment in 16SrRNA gene and a 375bp fragment in lipoprotein gene. The PCR evaluations were performed for
both the clinical samples and the cultures. Out of the 39 samples, 29 (74.3%) of the cultures were shown positive
and typical Mycoplasma colonies grew on PPLO agar, which could be considered as the diagnostic method. In
addition, 38 (97.4%) samples had positive PCR results for Mycoplasma genus and six (15.3%) of the samples
were shown to be positive using PCR for M. agalactiae as the diagnostic method. In the present study, M.
agalactiae was detected in semen of goat bulks for the first time in Iran. Therefore, it is recommended to
concern semen as one of the significant sources for this pathogen and the possibility for transmission to the
female goats through semen is highlighted. Moreover, presence of this microorganism in semen could be
involved in infertility of goat population.

Keywords

Main Subjects


Article Title [French]

L'isolement et l'identification du Mycoplasma agalactiae à partir de sperme de bouc

Abstract [French]

La maladie agalactie est l'une des maladies les plus infectieuses chez les moutons et les boucs. Cette maladie est dans la catégorie des infections à mycoplasme et est habituellement endémique. Mycoplasma agalactiae est la principale cause de cette maladie chez les boucs. Le but de cette étude était l'isolement et l'identification du Mycoplasma agalactiae à partir du sperme de bouc. Au total, 39 échantillons de sperme de boucs ont été prélevés. Une culture cellulaire a été effectuée sur tous les échantillons dans un milieu de culture adapté et spécifique (PPLO Broth) au Mycoplasma agalactiae. Après extraction de l'ADN des bactéries, des tests PCR ont été menés sur tous les échantillons en utilisant des amorces spécifiques à une séquences du gène 16S rRNA de 163 pb de longueur pour l’identification du genre Mycoplasma et une deuxième de 375 pb composant une partie du gène d’une lipoprotéine spécifique à l’espèces Mycoplasma agalactiae. Sur un total de 39 échantillons analysés, 29 échantillon (74.3%) étaient positifs dans le mileu de culture PPLO. Nos analyses par PCR spécifique au genre Mycoplasma ont révélé que 38 des 39 échantillons (97.4%) étaient positifs parmi lesquels 6 (15.3%) étaient également positifs au test PCR spécifique à l’espèce Mycoplasma agalactiae. Ce travail représente la première étude sur l'isolement et l'identification du Mycoplasma agalactiae présent dans le sperme des boucs en Iran. Les résultats de cette étude ont montré que le sperme pourrait être un endroit approprié pour la présence de cet agent pathogène et sa transmission à la femelle. Par conséquent, ce facteur peut être considéré comme l'une des causes potentielles d'infertilité chez les chèvres.

Keywords [French]

  • boucs
  • gène lipoprotéine
  • Mycoplasma agalactiae
  • Sperme
  • 16S rRNA
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