Infectious Bovine Rhinotracheitis is a highly contagious disease caused by the bovine herpes virus-1 (BoHV-1), resulting in significant losses to livestock around the world. BoHV-1 is a major pathogen of cattle, primarily associated with respiratory/genital tract infections and abortion. In the present study, we determined the presence of antibodies in 120 serum samples of cattle with the history of abortion in different period of pregnancy from different industrial dairy herds in Mashhad. Also we tested 30 samples from normal cattle with no history of abortion as negative control. The presence of antibodies against infectious bovine rhinotracheitis was investigated by enzyme-linked immunosorbent assay (ELISA). The results showed that seroprevalence of IBR in aborted cattle were 70% (84 samples). From these positive samples, 11 (13.09%), 42 (50.00%) and 31 (36.91%) samples were associated to the first, second and third trimester of pregnancy, respectively. From these seropositive cattle (84 samples), 12 (14.28%) samples were associated with stillbirth and 7 (8.33%) samples were related to mummified fetus. From 84 positive samples, 59 (70.24%) were related to cattle between 2-5 years old and 25 (29.76%) were associated to cattle more than 5 years old. In negative control group, 5 samples showed antibody against IBR antigen.