To evaluate the effect of intrauterine cephapirin on treatment of endometritis 39 Holstein dairy cows that affected with postpartum endometritis were selected and randomly assigned to two experimental designs. In experiment 1, 6 out of 14 cows were 25 to 30 days after parturition were treated by intrauterine oxytetracycline. Another 8 cows were treated by intrauterine cephapirin (Metri-Care). In experiment 2, 25 cows that were 55-60 days after parturition received three types of treatment. 6 cows were received only PGF2α, 9 cows were received PGF2α and procaine penicillin G, and 10 cows were treated by intrauterine Metri-Care. Cervical mucosa was collected before and after each treatment. There was no significant difference between mean (±SD) of neutrophils before and 14 days after treatment with them. Conception rates of cows received oxytetracycline and Metri-Care were 66.66% (4/6) and 37.5% (3/8), respectively. There was significant difference between mean of neutrophils before and 14 days after treatment at all groups. Conception rate of cows received only PGF2α was 33.33 % (2/6) and in cows received penicillin and PGF2α was 55.55% (5/9) but in cows received Metri-Care was 90.0% (9/10). Therefore, Metri- care may be good choice for treatment of postpartum uterine infection in cow.