Twelve (Experiment I) and four (Experiment II) multiparous dairy cows seronegative to pestivirus were selected and randomly assigned to either a control group which did not become infected or a treatment group in which all cows became infected following intranasal inoculation 9 days before AI. The experimental induction of infection was carried out with 2 ml of non-cytopathic pestivirus (BVD virus) suspension containing 5 log10 TCID50/ml (Experiment I) and 4.5 log10 TCID50/ml (Experiment II). In both experiments, the cows were superovulated on day 10±2 of the cycle using the standard procedures. The cows in Experiment I were artificially inseminated at 12 and 24 h after the onset of estrus and a non-surgical ova/embryo collection was performed 7 days after AI. In Experiment II, the cows were slaughtered on day 8 after superovulation-induced estrus and the ovaries submitted for gross and histopahological examination including immunohistochemistry. Mean (±SE) number of ovulatory sized follicles on day of AI and corpora lutea palpated on day 7 after AI were significantly (p
Bielefeldt-Ohmann, H. , McGowan, M. and Kafi, M. (2006). Bovine pestivirus infection: a cause of ovulatory disturbance in dairy cows. Archives of Razi Institute, 61(2), 97-104. doi: 10.22092/ari.2006.103789
MLA
Bielefeldt-Ohmann, H. , , McGowan, M. , and Kafi, M. . "Bovine pestivirus infection: a cause of ovulatory disturbance in dairy cows", Archives of Razi Institute, 61, 2, 2006, 97-104. doi: 10.22092/ari.2006.103789
HARVARD
Bielefeldt-Ohmann, H., McGowan, M., Kafi, M. (2006). 'Bovine pestivirus infection: a cause of ovulatory disturbance in dairy cows', Archives of Razi Institute, 61(2), pp. 97-104. doi: 10.22092/ari.2006.103789
CHICAGO
H. Bielefeldt-Ohmann , M. McGowan and M. Kafi, "Bovine pestivirus infection: a cause of ovulatory disturbance in dairy cows," Archives of Razi Institute, 61 2 (2006): 97-104, doi: 10.22092/ari.2006.103789
VANCOUVER
Bielefeldt-Ohmann, H., McGowan, M., Kafi, M. Bovine pestivirus infection: a cause of ovulatory disturbance in dairy cows. Archives of Razi Institute, 2006; 61(2): 97-104. doi: 10.22092/ari.2006.103789
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