Determination of Antibody Response against Inactivated Agalactia Vaccine in Small Ruminants


1 Proteomics and Biochemistry Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

2 Department of Microbiology, School of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran.


  An inactivated agalactia vaccine was prepared with Mycoplasma agalactiae in fermenter. The culture was inactivated and subsequently adjuvanted with aluminum hydroxide gel. Four sheep and four goats were immunized with the experimental vaccine. Two doses of 1ml of vaccine were administered three weeks interval. Monthly, blood samples were taken from the vaccinated animals up to the fifth month along with hyperimmune and negative controls. Elisa was set up and sera were assayed against Lorestan live antigen. Results indicated that antibodies were determined in vaccinated sheep and goats with the maximum overall mean titres of 1.25 and 1.028, at one month post-vaccination and minimum mean titres of 0.674 and 0.538 respectively.