Razi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601Genotyping of Different Pestiviruse Isolates by RT-PCRژنوتایپینگ جدایه های مختلف پستی ویروس توسط RT-PCR1810382010.22092/ari.2004.103820ENR. Kargar MoakharM.A. AkhavizadeganF. HemmatzadehF. AminiJournal Article201601121320 blood samples were collected from herds showing clinical signs of pestiviral infections. 39 samples were positive in Ag-ELISA assay. All these 39 samples in addition to 5 cytopathic strains were cultured in MD-BK cell line and presence of pestiviral antigens was confirmed by direct-immunofluorescent test. 27 out of 44 BVDV suspected isolates were detected by RT-PCR using a primer set. Differentiation among the viruses was achieved by cutting the PCR products with restriction endonucleses enzymes Ava1, Bgl1 and Alu1. Using this procedure it was possible to distinguish at least two genogroups, 1 and 2 BDV containing 14 and 3 isolates, respectively.https://archrazi.areeo.ac.ir/article_103820_5c10786d4a99bf90f942ee72884bd3f9.pdfRazi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601Pathogenicity Study and Restriction Enzyme Profile of a Recently Isolated Infectious Bursal Disease Virus in Iranمطالعه آسیبزایی و پروفایل محدودیت آنزیمی یک ویروس بیماری بورس عفونی تازه جدا شده از ایران91810382110.22092/ari.2004.103821ENA.H. ShoshtariS.A. PourbakhshH.A. DadrasM.A. BahmaninejadR. ToroghiJournal Article20160112The pathogenicity of a recent isolate of Infectious bursal disease virus (IBDV), IR499, isolated from a nonvaccinated flock with 17.5 % mortality rate in susceptible SPF chickens, chickens embryos and broilers was discussed. The molecular characterization of the virus based on the restriction fragment length polymorphism (RFLP) pattern was also investigated. The mortality rates were 85% and 22% in SPF and broiler chickens, respectively. The bursal weight indexes were 4.7 and 1.2 for SPF birds and 1.7 and 0.7 for susceptible broilers four and nine days post inoculation, respectively. The gross lesions were generalized including moderate to sever bursal hemorrhage. Using RT-PCR, a 643bp PCR product was amplified then nested PCR was carried out to amplify a 552bp PCR product. The 552bp product was subjected to SspI, StuI, HhaI and SacI restriction enzymes digestion which was SspI and StuI double positive and HhaI and SacI double negative. The pathogenicity and RFLP pattern finding confirmed that the IR499 virus could be classified as a very virulent IBDV.https://archrazi.areeo.ac.ir/article_103821_70664e3611d9cf621347381e4de1b220.pdfRazi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601Expression and Purification of Recombinant Outer Surface Protein D of Borrelia burgdorferiبیان و خالص سازی پروتئین D سطح خارجی نوترکیب Borrelia burgdorferi192710382210.22092/ari.2004.103822ENM.H. SanatiF. AlastiH. AleyasinM. MostafaviP.R. CarnegieJournal Article20160112To carry out the immunological experiments on the serum of Multiple Sclerosis (MS) patients, based on a correlation between Borrelia burgdorferi infection and contracting MS autoimmune disease the outer surface protein D (OspD) of the bacterium was expressed and purified. A clone containing the OspD gene in pET11a expression vector under the control of T7 promoter was transformed to the bacterial host BL21 (DE3). Some of the colonies were selected for IPTG-induced expression. The colony with the highest amount of OspD was selected for large-scale expression. Large-scale protein purification was performed by the reversed phase HPLC with a C4 preparative column; ultimately, the expressed purified protein was confirmed by the Western blot technique.https://archrazi.areeo.ac.ir/article_103822_34a50780b9ff921897045fcc43b147b7.pdfRazi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601Isolation of Anthrax Spores from Soil in Endemic Regions of Isfahan, Iranجداسازی هاگهای شاربن از خاک مناطق بومی اصفهان، ایران293810382310.22092/ari.2004.103823ENG.R. Moazeni JulaA.R. JabbariB. MalekJournal Article20160112To isolate and detect anthrax spores from soil in different regions of Isfahan, Iran a total of 60 environmental specimens were collected during 2003. Bacterial endospores were extracted via flotation in distilled water and were cultured on blood agar and selective PLET media. Bacillus anthracis was identified using bacteriological and biological tests. Viable Bacillus anthracis spores were isolated from 9 (15%) soil samples of the 60 collected specimens in which 6 (66%) of isolates were encapsulated. The isolated bacteria and their virulence were confirmed with polymerase chain reaction (PCR) using specific primers. Its recommend that because of the existence of highly virulent strain of Bacillus anthracis in this region, a review on implementation of control programs such as regular vaccination of all susceptible livestock and surveillance of the disease in animals and human in such endemic areas is required.https://archrazi.areeo.ac.ir/article_103823_d429173e46a63af7efc8351712cef579.pdfRazi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601Isoelectric Focusing and PCR-RFLP Joined Techniques for Alpha1-antitrypsin Deficiency Detectionتمرکز ایزوالکتریک و روشهای متصل به PCR-RFLP برای کشف کمبود Alpha1-antitrypsin394910382410.22092/ari.2004.103824ENB.Gh. GhoudarziA. LotfiA. MesbahA. Zare MirakabadiR. BagherianJournal Article2016011253 persons suspected to alpha1-antitrypsin deficiency detection (AATD) were investigated for ZZ, MZ, ZS, SS, and MS alleles analysis by serum protein electrophoresis (SPE), measurement of trypsin inhibiting capacity (TIC), isoelectric focusing (IEF), polymerase chain reaction (PCR), and IEF/PCR-RFLP techniques. The result clearly shows by using SPE and TIC techniques only 35.85 % and 50.08% of AATD cases can detect respectively while, IEF/PCR-RFLP joined technique can detect 100% of AATD cases. However, since IEF/PCR-RFLP joined technique can determine the protein structure and alleles gene mutation together, they can detect up to 100% of AAT. Hence for detection of AATD the IEF/PCR-RFLP technique is recommended.https://archrazi.areeo.ac.ir/article_103824_989a9a609779334dc3ee7b9502bfda3c.pdfRazi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601Histopathological Study of Intratracheally Inoculated A/Chicken/Iran/259/1998 (H9N2) Influenza Virus in Chickenمطالعه هیستوپاتولوژیکی ویروس آنفلوانزای تلقیح شده داخل نای (H9N2) در جوجههای مرغ516210382510.22092/ari.2004.103825ENS.A. PourbakhshI. Sohraby HaghdostM.H. HablolvaridM.R. GholamiJournal Article20160112https://archrazi.areeo.ac.ir/article_103825_3d56ffa45fac1e202f843547dd3db366.pdfRazi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601Proliferate Resonse to Purified Bordetella pertussis Toxin on Murine Spleen Lymphocytesپاسخ تکثیری به توکسین خالص شده Bordetella pertussis روی لمفوسیتهای طحال موشها637110382610.22092/ari.2004.103826ENA. PourahmadiF. EsmailiA. Zavaran HosseiniA. RezaeeE. SalehiM.A. AkhavizadeganA. MirjaliliJournal Article20160112A purification procedure of pertussis toxin (PT) from submerged culture of Bordetella pertussis (B.pertussis) strain 134 using adsorption and affinity chromatography was discussed. The yield of the resulting PT was approximately 37.5mg/l of concentrated culture supernatant. The polypeptide pattern of the purified PT was investigated by SDS-PAGE and showed five bands between 11 to 26 KDa using low molecular weight marker. Since PT is the main component of acellular pertussis vaccine, obtaining a good yield of it would be essential for production of the new and safer vaccine generation. The other objective of this study was to determine the effects of PT on murine lymphocytes using MTT test. The effect of various doses of prepared PT on murine lymphocytes showed that the amount of 0.5μg/0.1ml had the highest proliferation. Furthermore comparison between the resulting PT and phytohemagglutinin showed much higher effect of PT on murine spleen cells. These results indicate that B.pertussis strain 134 is a suitable strain for induction of PT in order to use it for development of acellular vaccine in Iran and also for in vitro studies on proliferation of murine lymphocytes.https://archrazi.areeo.ac.ir/article_103826_3039ccb92f6e2739cc680a52cafb4ec5.pdfRazi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601The Effect of Mafosfamide on Differential Activation of T-helper Subsetsاثر Mafosfamide بر فعال سازی تمایزی زیرمجموعه های T-helper738310382710.22092/ari.2004.103827ENE. AsliM.J. DascombeA.V. HutchinsonM. TebianianR. SadriJournal Article20160112https://archrazi.areeo.ac.ir/article_103827_6587ec9ebe50f514e9c954fc42fde6aa.pdfRazi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601The dsRNA Electrophoretype of Some Isolated Iranian Calf Rotavirusesالکتروفورز dsRNA برخی از روتاویروسهای جدا شده گوساله در ایران858910382810.22092/ari.2004.103828ENM. GhorbanpourH. KeyvanfarM. Seify-abad ShapouriJournal Article20160112A rapid and simple technique for diagnosis of rotaviral diarrhea is polyacrylamide gel electrophoresis (PAGE) of dsRNA extracted from fecal samples. To determine the electrophoretype of Iranian calf rotavirus, 23 rotaviral positive fecal samples from diarrheic Holstein calves at the age of less than one month in Tehran region were examined. The viral dsRNA was extracted with phenol-chloroform, and analyzed by PAGE and silver staining. According to PAGE all of the electrophoretypes were identified as long genome electrophoretypes, characteristic of animal group A rotavirus. There was not any unusual segment rearrangement.https://archrazi.areeo.ac.ir/article_103828_6b7ea6db920443c61bb339621e50d40d.pdfRazi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601A Pathologic and Microbiologic Study on Bovine Arthritis Associated with Mycoplasma spp.مطالعه آسیبشناسی و میکروبشناسی تورم مفصل گاوان ایجاد شده با گونههای مختلف مایکوپلاسما9710410382910.22092/ari.2004.103829ENA.H. TabatabayiJ. NajafiM.J. GharagozlouP. KhazrainiaJournal Article201601123450 bovine from different breeds and ages were clinically examined for the presence of arthritis. 120 cases showed macroscopic evidences of arthritis. Mycoplasma spp were isolated from synovial fluid of 21 affected animals. In addition to Mycoplasma spp. Arcanobacterium pyogenes and Staphylococcus epidermidis were isolated from 2 cases. The synovial fluid was markedly increased, straw color, turbid and mostly contained thick confluent fibrin. Thickening of the synovial membrane, villus hyperplasia, loss of the synovial cells, infiltration of leukocytes, hyperemia and edema of synovial membrane and periarticulare tissues were seen. Isolation of Mycoplasma from the bovine with arthritis indicates the importance of the organism as causative agent of the disease.https://archrazi.areeo.ac.ir/article_103829_a23ffb8de45e948bf4c67ce0aeea9654.pdfRazi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601The Effect of Colistin Sulfate in Feed on the Controlling of Salmonella enteritidis Contamination in a Broiler Farmتاثیر کلیستین سولفات داخل دان بر روی کنترل آلودگی با سالمونلا انتریتیدیس در یک مزرعه پرورش مرغ گوشتی10511010383010.22092/ari.2004.103830ENM.H. Bozorgmehri FardJournal Article20160112The effect of colistin sulfate on reducing Salmonella enteritidis (S.enteritidis) infection in broilers and contamination from broiler carcasses was evaluated. 40000 birds in two separate houses were considered. Colistin sulfate was added in to the feed of the test group as 100g containing 1,200,000IU/ton of feed for the whole period (56 days). To isolate S.enteritidis, samples were taken from different parts of the intestine and cultured in Selenite broth and then on SS agar plates. The suspected colonies were isolated and identified by biochemical and serological tests. It is conducted that the addition of the above mentioned amount of this antibiotic in to the broiler feed could decrease the rate of the infection of flocks and contamination of carcasses with S.enteritidis. The results also indicate that due to addition of colistin sulfate, the live weight gain increases by 14% and the feed conversion rate improves by 8% in this study.https://archrazi.areeo.ac.ir/article_103830_184938614a774cab604161489b6d37e1.pdfRazi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601The Rapid CAMP Test for Identification of Streptococcus agalactiae Using Alpha Toxinتست سریع CAMP برای شناسایی Streptococcus agalactiae با استفاده آلفاتوکسین11912410383110.22092/ari.2004.103831ENA. KhafriA. NazariJournal Article20160112Alpha toxin derived from Clostridium perfringens was used for diagnosis of Streptococcus agalactiae (St.agalactiae) by rapid and spot CAMP tests. The selected Cl.perfringens strain was cultured in optimized conditions; the supernatant of culture was concentrated by ultra filter and purified by DEAE cellulose chromatography. The efficacy of both purified and crude α-toxins were examined by rapid and spot CAMP tests. The result of this study indicates that there is no significant difference between the purified and crude enzyme in identification of St.agalactiae. The specificity of the enzyme was confirmed by testing of different Streptococcus spp. The run time require for spot and rapid CAMP tests using α-toxin were between 10-90 minutes and 4-6 hours, respectively, whereas the traditional test needs 18-24 hours for running.https://archrazi.areeo.ac.ir/article_103831_79c4143432343c91921c8cf96b2c71c3.pdfRazi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601Population Density,Trematodal Infection and Ecology of Lymnaea Snails in Shadegan, Iranتراکم جمعیتی، آلودگی ترماتودی و اکولوژی حلزونهای لیمنه در شادگان ایران12512910383210.22092/ari.2004.103832ENG.R. KarimiM. DerakhshanfarH. PaykariJournal Article20160112Two snail species belonging to genus Lymnaea, L.auricularia and L.gedrosiana, were collected from 6 different districts of Shadegan area of Khoozestan. Eight percent of the total collections of the snails revealed the occurrence of larva in them. The prevalence of larva within the snails varied in different districts may be due to different geoclimatic conditions and availability of fresh water. Seasonal prevalence of snails and factors affecting snail populations including water temperature, light, water depth and pH under field conditions were also studied.https://archrazi.areeo.ac.ir/article_103832_cf0a3bfc6c8ab9b567a6170c4c1b4102.pdfRazi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601Virulence of Avian Serotype A1 Pasteurella multocida for Chickens and Mice.حدت پاستورلا مولتوسیدا سروتیپ A1 برای جوجه ها و موشها919610383310.22092/ari.2004.103833ENA. SotoodehniaS. AtaieG.R. MoazeniA.R. JabbaeiM. TabatabaieJournal Article20160112The virulence of Pasteurella multocida (P. multocida) serotype A1 for chickens and mice was determined. Groups of chicken and mice were exposed intramuscularly and intraperitoneally to various concentration of P. multocida broth culture, respectively. This strain was highly virulent for chickens so that those exposed to only 7 c.f.u. of the organism died in less than 24 hours. Groups of mice exposed to the virulent strain died during 48 hours post inoculation. Microbiological examination resulted in the isolation of P. multocida from exposed chicken and mice. No isolation was made from unexposed control groups. The result indicates that the isolate is highly virulent for both chickens and mice.https://archrazi.areeo.ac.ir/article_103833_528ea478948d6e4b7bc01494b2157d5d.pdfRazi Vaccine & Serum Research InstituteArchives of Razi Institute0365-343958120040601Antibiotic Sensitivity of Ornithobacterium rhinotracheale Isolates Associated with Respiratory Diseases.حساسیت آنتی بیوتیکی جدایه های Ornithobacterium rhinotracheale مرتبط با بیماریهای تنفسی11111710383410.22092/ari.2004.103834ENM. BananiS.A. PourbakhshA.H. DeihimiJournal Article20160112187 commercial checken flocks affected with respiratory diseases were examined for Ornithobacterium rhinotracheale isolation. The bacterium was isolated from 105 (56.2%) poultry flocks. Drug sensitivity test using standard disk diffusion technique was performed with 19 antibiotics. All the isolates were susceptible to tiamulin and most of them were susceptible to chloramphenicol and linco-spectin. All the isolates were resistant to sulfamethoxazol-trimethoprim, colistin and neomycin and most of them were resistant to gentamycin, lincomycin, erythromycin, tetracycline and enrofloxacin. One isolate from a native turkey was also tested. This isolate was resistant to sulfamethoxazole-trimethoprim, colistin, neomycin and gentamycin, but was sensitive to other tested antimicrobials. Because of acquired antibiotic resistance, the various result of antibiotic therapy, it must be stressed to prevent the infection.https://archrazi.areeo.ac.ir/article_103834_7a8ab95e47aea71cf9e2b265944b4fb1.pdf