Razi Vaccine & Serum Research Institute Co-Publisher: Negah Scientific Publisher Archives of Razi Institute 0365-3439 78 6 2023 12 01 First Isolation of Bacillus licheniformis from Bovine Mastitis in Iran اولین جداسازی باسیلوس لیکنی فورمیس از ورم پستان گاوی در ایران 1690 1697 129051 10.32592/ARI.2023.78.6.1690 EN B Nayeri Fasaei Department of Microbiology and Immunology , Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran I Ashrafi Tamai Department of Microbiology and Immunology , Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran S M Joghataei Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Journal Article 2023 03 05 <em>Bacillus licheniformis</em> is a gram-positive, endospore-forming, saprophytic and facultative anaerobe that is resistant to heat and environmental conditions. This study was the first to isolate and confirm <em>B. licheniformis</em> as a cause of bovine mastitis in Iran.<strong> </strong>In the summer of 2020, 105 samples of mastitic milk were collected from dairy farms around Tehran and sent to the microbiology laboratory of the Faculty of Veterinary Medicine at the University of Tehran. The bacterial pathogens were identified using selective and differential culture media and confirmed by PCR to contain the toxin synthetase genes <em>lic</em>A, <em>lic</em>B and <em>lic</em>C in mastitic isolates of <em>B. licheniformis</em>. Resistance patterns to 19 antibiotics were determined for two isolates of <em>B. licheniformis</em>.<strong> </strong><em>Staphylococcus aureus</em> and <em>Escherichia coli</em> were identified as the most important organisms in the samples. <em>B. licheniformis</em> was isolated from the two samples containing all three genes. Both isolates were resistant to streptomycin, trimethoprim-sulfamethoxazole, cefixime, ampicillin, bacitracin, clindamycin, and gentamicin.<strong> </strong><em>B. licheniformis</em> was reported for the first time in Iran as a cause of bovine mastitis with clinical symptoms. The first isolation of toxin-producing strains of <em>B. licheniformis</em> from mastitic cows in Iran raises concerns about the safety of dairy products. In principle, selected strains with toxigenic potential should not be used as feed additives and animal feed. However, whole genome sequencing is proposed to search for genes coding for toxins. https://archrazi.areeo.ac.ir/article_129051_5cf6786e33a7f376e33050a207e4d0a1.pdf