Razi Vaccine & Serum Research Institute Co-Publisher: Negah Scientific Publisher Archives of Razi Institute 0365-3439 77 6 2022 12 01 Development of Resource-Saving Biotechnology for the Production of a Feed Additive from Distiller’s Wastes with Probiotic Properties 2281 2289 127827 10.22092/ari.2022.360053.2536 EN Z Alimkulo Head of the Laboratory of Grain and Feed Technology, Kazakh Research Institute of Processing and Food Industry, LLP, 050060, Kazakhstan, Almaty, Gagarin str., 238 A, Kazakhstan M Velyamov Head of the Laboratory of Biotechnology, Quality and Food Safety, Kazakh Research Institute of Processing and Food Industry, LLP, 050060, Kazakhstan, Almaty, Gagarin str., 238 A, Kazakhstan I Potoroko Head of the Department of Food and Biotechnology of the Higher Medical and Biological School, Federal State Autonomous Educational Institution of Higher Education, South Ural State University, (NRU), 454080, Russia, Chelyabinsk, Lenin Ave. 76, Kazakhstan S Kuanysh Almaty Technological University, 050000, Kazakhstan, Almaty, Tole bi str., 100, Kazakhstan T Zhumaliyeva Biotechnology, Almaty Technological University, 050000, Kazakhstan, Almaty, Tole bi str., 100, Kazakhstan K Shauliyeva Kazakh Research Institute of Processing and Food Industry, LLP, 050060, Kazakhstan, Almaty, Gagarin str., 238 A, Kazakhstan Journal Article 2022 08 18 The trend towards an increase in ethanol production on a global scale and the tightening of restrictive measures regarding the disposal of by-products from production increases the relevance of research in finding ways to process them. This study aimed to assess the effectiveness of a feed additive containing dried stillage fermented with pre-immobilized cultures of Lactobacillus pontis 67, Lb. casei 22, Lb. paracasei 104 when finishing steers. The dose of inoculum (3.0%) of a liquid probiotic drug based on the consortium Lb. pontis 67, Lb. casei 22, Lb. paracasei 104 was determined, cultivation time 24-30 hours at a bacterial titer of 10-10 CFU/ml, and the characteristics of fermented stillage and a feed additive based on it were established. Studies were conducted to assess the effectiveness of finishing when using fermented wheat stillage on the qualitative characteristics of the carcass of 13-month-old Kazakh white-headed steers on finishing. The studies were conducted in 2 groups: control and experimental (13% fermented stillage). The experimental group showed higher indicators of average daily gain (Р˂0.05), carcass weight, and slaughter yield (P˂0.05). According to the results of the conducted research, it can be concluded that the replacement of protein components of compound feed with stillage in an amount of 10% and a probiotic drug based on the bacteria Lb. pontis 67, Lb. casei 22, Lb. paracasei 104 in an amount of 3.0% contributes to an increase in weight gain when finishing steers by 9.1±0.3 kg, average daily gain by 417±2.0 g, and slaughter yield by 3.1±0.2% compared with the control group (P˂0.05).

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