Razi Vaccine & Serum Research Institute Co-Publisher: Negah Scientific Publisher Archives of Razi Institute 0365-3439 62 2 2007 06 01 A serological survey for hydatidosis among buffaloes in Orumia بررسی سرولوژیک کیست هیداتیک در جمعیت گاو میش شهرستان ارومیه 101 104 103775 10.22092/ari.2007.103775 EN S. Navidpour N. Abshar Gh.R. Motamedi Gh.R. Karimi H. Paykari Journal Article 2016 01 12 Hydatid cyst is an important and zoonotic infection caused by cystic stage of Echinococcus granulosus. It is a major and economic problem in most areas of the world that livestock are kept such as Iran. In this study, a total of 111 buffalo,s sera from Center of Buffalo Sperm Preparation (CBSP), Orumia (West Azarbayjan province, Iran, 2003) were examined for the presence of hydatidosis by using enzyme-linked immunosorbent assay (ELISA). 5 μg protein per mL of antigen B from sheep hydatid cyst fluid was used in this assay. Optimum dilution for rabbit anti-bovine peroxidase conjugate (Sigma) was used 1:5000. Overall results indicated 32.4% of serum samples positive for hydatidosis. Results indicate buffalo is sensitive intermediate host for Echinococcus granulosus in this province. https://archrazi.areeo.ac.ir/article_103775_2600bb60538db1450460750df2e17554.pdf