TY - JOUR ID - 126387 TI - Isolation and Molecular Detection of Feline Infectious Peritonitis Virus JO - Archives of Razi Institute JA - ARI LA - en SN - 0365-3439 AU - Mohammed Ibrahim, O AU - Bara Allawe, A AU - Ali Kadhim, H AD - Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq AD - Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq Y1 - 2022 PY - 2022 VL - 77 IS - 5 SP - 1709 EP - 1714 KW - Cell culture KW - Feline coronavirus KW - Feline infectious peritonitis virus KW - Real-time RT-PCR DO - 10.22092/ari.2022.357997.2135 N2 - Feline coronavirus (FCoV) is an enveloped single-stranded RNA virus, affecting wild and domestic cats. Feline infectious peritonitis viruses (FIPV) variants of FCoV cause fatal peritonitis affecting approximately 5% of FCoV infected animals. The present study aimed to detect and isolate the feline infectious peritonitis virus for the first time in Iraq. In this study, 50 samples (fecal swab and peritoneal fluid) were collected from suspected pet cats from different areas of Baghdad, Iraq. The very suitable age was under two years old.  Real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) was used to detect Feline infectious peritonitis in infected collected samples by the amplification of spike protein (S). The result of real-time RT-PCR revealed that out of 50 samples from suspected cats, 10 samples were positive for FIPV. Moreover, 10 positive samples by real-time RT-PCR were used for the isolation of the virus in chicken embryo fibroblast cell culture. Subsequently, the isolated virus was detected by real-time RT-PCR and then by conventional RT-PCR, followed by electrophoresis. UR - https://archrazi.areeo.ac.ir/article_126387.html L1 - https://archrazi.areeo.ac.ir/article_126387_de1dde91b3c6a21e1e72a171e60600f9.pdf ER -