TY - JOUR ID - 121544 TI - Prevalence and Early Detection of Hypodermosis in Goats using a Competitive ELISA System in Lorestan, Iran JO - Archives of Razi Institute JA - ARI LA - en SN - 0365-3439 AU - Bagheri, A AU - Madani, R AU - Navidpour, Sh AU - Hoghoooghi-Rad, N AD - Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran AD - Proteomics and Biochemistry Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran AD - Department of Venomous Animal and Antivenom Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran Y1 - 2021 PY - 2021 VL - 76 IS - 1 SP - 69 EP - 77 KW - ELISA KW - Goat KW - Przhevalskiana KW - Lorestan DO - 10.22092/ari.2019.125071.1296 N2 - This study aimed to determine the prevalence and early detection of hypodermosis in goats by the investigation of Przhevalskiana larvae and sera collected from the infested animals. This study was conducted in Lorestan province, located in the South-West of Iran, from April 2017 up to April 2018. A total of 3350 goats slaughtered in Lorestan abattoirs were investigated by clinical-parasitological examinations in different periods. The larvae were collected from the back and flank regions of the slaughtered goats. The number of infested animals, gender and age, number of maggots present on the body of each animal, location, and larval stage of warble flies were recorded in this study. To detect an infestation in the early period, a total of 150 blood samples were randomly collected from the field animals in Lorestan, Iran. The morphological findings showed that out of 3350 goats examined, 706 (21.07%) goats were infested. Furthermore, three species of Przhevalskiana, including P. Silenus (n=726, 50.07%), P.crossii (n=440, 30.43%), and P. aegagri (n=284, 19.59%) were recognized as the causative agents of goat hypodermosis in this province. No significant difference was observed between genders and/or among the age groups (P>0.05). The anti-Przhevalskiana antibodies in the serum samples were detected using ELISA from August up to mid-September (summer). Clinical diagnosis of infestation was usually performed from late October until mid-March (winter) by visual observations and direct palpation of warbles in the back and flank regions of the animals. It could be concluded that the use of ELISA can help to detect hypodermosis among goats in the early stages. UR - https://archrazi.areeo.ac.ir/article_121544.html L1 - https://archrazi.areeo.ac.ir/article_121544_a3543da9d18ceb67722daaecd64d1d6d.pdf ER -