TY - JOUR ID - 116399 TI - Diagnosis of Avian Mycoplasmas: A Comparison between PCR and Culture Technique JO - Archives of Razi Institute JA - ARI LA - en SN - 0365-3439 AU - Muhammad, F. AU - Hussain, J. AU - Fareed, S. K. AU - Ahmad Khan, T. AU - Ahmad Khan, S. AU - Ahmad, A. AD - Department of Microbiology, Faculty of Science, University of Karachi, Karachi, Pakistan AD - Department of Physiology, Faculty of Science, University of Karachi, Karachi, Pakistan Y1 - 2018 PY - 2018 VL - 73 IS - 3 SP - 239 EP - 244 KW - Avian Mycoplasmosis KW - Mycoplasma gallispticum KW - Mycoplasma synoviae KW - PCR KW - Culture DO - 10.22092/ari.2017.108217.1085 N2 - Mycoplasma gallisepticum and Mycoplasma synoviae are the causative agents of avian mycoplasmosis in commercial poultry. Among the available tools, polymerase chain reaction (PCR) and culture are confirmatory tools for the diagnosis of mycoplasmosis after the initial serological screening of suspected birds. Overall, 181 samples were analyzed, 152 (84%) and 103 (57%) of which were found positive by PCR and culture, respectively. Further, 54 (92%) broiler samples were found positive for general avian mycoplasma. Among the total positive samples, MS positivity was as high as 72 (47%) by PCR, while it was 45 (44%) by culture. MG positivity was 23% and 25% in PCR- and culture-positive samples. MG grows more easily compared to MS. The agreement value between the tests was 67%. Overall, flock wise prevalence was not much varied. The prevalence of mycoplasmosis was higher during winter. Our study confirmed that PCR is the most sensitive and reliable tool for the diagnosis of avian mycoplasmosis in field samples. UR - https://archrazi.areeo.ac.ir/article_116399.html L1 - https://archrazi.areeo.ac.ir/article_116399_e7a02d32be459e70722f1417b1804def.pdf ER -