TY - JOUR ID - 109843 TI - Prevalence of adhesion Virulence factor genes, antibiogram, and pathogenicity of avian Pasteurella multocida isolate from Iran JO - Archives of Razi Institute JA - ARI LA - en SN - 0365-3439 AU - Haghnazari, Sepideh AU - Jabbari, Ahmad Reza AU - Tadayon, Keyvan AD - Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran AD - Pasteurella National Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran AD - Department of Aerobic Bacterial Vaccine Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran Y1 - 2017 PY - 2017 VL - 72 IS - 2 SP - 83 EP - 91 KW - Pasteurella multocida KW - adhesion factors KW - antibiogram KW - pathogenicity KW - fowl cholera DO - 10.22092/ari.2017.109843 N2 - Pasteurella multocida possesses various virulence factors, including capsule, lipopolysaccharide, fimbriae, toxins, outer membrane proteins, and adhesions. Adhesins have a crucial role in mediating colonization and invasion of the host. The aim of the present study was to identify the prevalence of adhesion factor genes and resistance/sensitivity patterns among the avian P. multocida isolates from Iran. A total of 30 isolates of P. multocida were used for this study. All isolates were obtained from the poultry cases dead from fowl cholera in the northern parts of Iran. The results of the polymerase chain reaction analysis for the frequency of virulence-associated genes showed that the genes encoding adhesins (i.e., ptfA, fimA, hsf-1, pfhA, and ompH) were found in all (100%) of the isolates. However, the frequency of two genes including tadD and toxA were 50% and 70%, respectively. Thegenotyping patterns were classified into four groups according to the virulence factors in P. multocida isolates. Genotype pattern I, which included the isolates harbouring all of the examined virulence factor genes showed the highest frequency (43.3%). Pathogenicity test showed that all of the isolates classified as genotype I were pathogen or highly pathogen in the mice model. The sensitivity of the isolates to penicillin, ampicillin, lincospectin, florfenicol, tylosin, and tiamulin was 100%. However, the sensitivity rates to flumequin, enrofloxacine, and nalidixicacid were 96.6% and 80%, respectively. The findings of the current study will be helpful to elucidate the disease process and develop an efficient multivalent local vaccine.   UR - https://archrazi.areeo.ac.ir/article_109843.html L1 - https://archrazi.areeo.ac.ir/article_109843_baab56bfaa009795f740ecba90cd7f16.pdf ER -