TY - JOUR ID - 105993 TI - Evaluation of cytokine mRNA expression in vaccinated guinea pigs with foot-and-mouth disease type O inactivated vaccine JO - Archives of Razi Institute JA - ARI LA - en SN - 0365-3439 AU - Pasandideh, R. AU - Nassiri, M.R. AU - Raouf Delgosha, M. AU - Aslaminejad, A.A. AU - Tahmoorespur, M. AU - Zibaei, S. AU - Doosti, M. AU - Pasandideh, M. AD - Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran AD - Razi vaccine and Serum Research Institute, Mashhad, Iran AD - Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran Y1 - 2016 PY - 2016 VL - 71 IS - 1 SP - 15 EP - 19 KW - Cytokine KW - mRNA expression KW - FMD KW - Guinea pig KW - Real-time PCR DO - 10.22034/ari.2016.105993 N2 - Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. This study quantifies the cytokines mRNA expression of vaccinated guinea pigs withFMD type O inactivated vaccine. Blood samples were collected from eight guinea pigs at 7 and 28 days after thefirst vaccination. Extracted mRNAs were reverse-transcribed into cDNA and analyzed for quantification of IFN-γ, TNF-α and IL-10 expression using relative real-time PCR assay. Our results showed that all of the genes wereupregulated. The expression of TNF-α and IL-10 genes significantly increased (P<0.05) in day 28th incomparison to the day 7th post the first vaccination. It can be concluded that the vaccine induced immuneresponses by increasing expression of the cytokines. Therefore, effects of DNA vaccines on immune system alsomay be evaluated using these genes. UR - https://archrazi.areeo.ac.ir/article_105993.html L1 - https://archrazi.areeo.ac.ir/article_105993_e358b281e06eba22dfbdbb7353b442cc.pdf ER -