TY - JOUR ID - 103988 TI - Preparation, characterization and stability investigation of chitosan nanoparticles loaded with the Echis carinatus snake venom as a novel delivery system JO - Archives of Razi Institute JA - ARI LA - en SN - 0365-3439 AU - poMohammadur Dounighi, N. AU - Mehrabi, M. AU - Avadi, M.R. AU - Zolfagharian, H. AU - Rezayat, M. AD - Department of Venomous animals and Human Sera, Razi Vaccine & Sera Research Institute, Karaj, Iran AD - Department of Nanotechnology, Faculty of Advanced Sciences and Technology in Medicine, Tehran University of Medical Science, Tehran, Iran AD - Research and Development Department, Hakim pharmaceutical company, Tehran, Iran AD - Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Y1 - 2015 PY - 2015 VL - 70 IS - 4 SP - 269 EP - 277 KW - chitosan nanoparticles KW - tripolyphosphate KW - Venom KW - Ionic gelation KW - Stability DO - 10.7508/ari.2015.04.007 N2 - The chitosan nanoparticles used widely as a drug delivery systems recently. In the present study the Echis carinataus snake venom loaded chitosan nanoparticles were prepared based on ionic gelation of tripolyphosphate and chitosan. The nanoparticles physicochemical characteristics, stability and biological activity of encapsulated venom were studied. The particles were spherical in shape and the tripolyphosphoric groups of TPP were linked to the ammonium groups of chitosan. Optimum particle size of chitosan and venom loaded nanoparticles were 89 and 116 nm, respectively. Optimum loading capacity and loading efficiency obtained by 500 µg/ml concentration of venom. The biological activity of venom remained intact during nanoparticle formation and showed no considerable reduction in stability analysis. Our results suggested that Chitosan nanoparticles, which prepared in this work possibly, could be used as an alternative for traditional adjuvants. UR - https://archrazi.areeo.ac.ir/article_103988.html L1 - https://archrazi.areeo.ac.ir/article_103988_5e9050c04027f7fc09f4d65d2213441b.pdf ER -